Literature summary for 2.4.1.B34 extracted from

Xu, X.; Dechesne, A.; Visser, R.G.; Trindade, L.M.
Expression of an (engineered) 4,6-alpha-glucanotransferase in potato results in changes in starch characteristics (2016), PLoS ONE, 11, e0166981 .

Search for more literature for 2.4.1.B34

Cloned(Commentary)

Cloned (Comment)	Organism
gene gftB, recombinant expression in Solanum tuberosum amylose-containing line Kardal and amylose-free mutant amf, the gene is cloned in frame with GBSSI transit peptide to allow amyloplast targeting and is driven by the potato GBSSI promoter for tuber expression, quantitative RT-PCR expression analysis	Limosilactobacillus reuteri

Protein Variants

Protein Variants	Comment	Organism
additional information	an (engineered) 4,6-alpha-glucanotransferase (GTFB) from Lactobacillus reuteri 121 is introduced into two potato genetic backgrounds: amylose-containing line Kardal and amylose-free mutant amf. The resulting starches show severe changes in granule morphology regardless of genetic backgrounds. Modified starches from amf background exhibit a significant increase in granule size and starch phosphate content relative to the control, while starches from Kardal background display a higher digestibility, but do not show changes in granule size and phosphate content. Transcriptome analysis reveal the existence of a mechanism to restore the regular packing of double helices in starch granules, which possibly results in the removal of novel glucose chains potentially introduced by the (engineered) GTFB. This amendment mechanics can also explain the difficulties to detect alterations in starch fine structure in the transgenic lines	Limosilactobacillus reuteri

Organism

Organism	UniProt	Comment	Textmining
Limosilactobacillus reuteri	A0A0U5F702	-	-
Limosilactobacillus reuteri 121	A0A0U5F702	-	-

Synonyms

Synonyms	Comment	Organism
4, 6-alpha-glucanotransferase	-	Limosilactobacillus reuteri
GtfB	-	Limosilactobacillus reuteri
UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase stabilizing protein	UniProt	Limosilactobacillus reuteri

General Information

General Information	Comment	Organism
physiological function	4,6-alpha-glucanotransferase from Lactobacillus reuteri strain 121 (GTFB) can convert starch or starch hydrolysates into isomalto/maltopolysaccharides (IMMPs). This enzyme can transfer the non-reducing glucose moiety of an alpha-1,4 glucan chain to the non-reducing end of another alpha-glucan through alpha-1,6 linkages, generating a linear chain with alpha-1,6 linkages. This specific activity makes GTFB an interesting target enzyme for producing distict starches in planta	Limosilactobacillus reuteri

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Release 2023.1 (January 2023)