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Literature summary for 2.4.1.B34 extracted from

  • Te Poele, E.; Corwin, S.; Hamaker, B.R.; Lamothe, L.; Vafeiadi, C.; Dijkhuizen, L.
    Development of slowly digestible starch derived alpha-glucans with 4,6-alpha-glucanotransferase and branching sucrase enzymes (2020), J. Agric. Food Chem., 68, 6664-6671 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.B34

Cloned(Commentary)

Cloned (Comment) Organism
gene gtfB, recombinant expression of C-terminally His-tagged enzyme mutant GtfB-DELTAN-DELTAV in Escherichia coli strain BL21(DE3) Limosilactobacillus reuteri

Protein Variants

Protein Variants Comment Organism
additional information construction of the GtfB-DELTAN-DELTAV mutant, a truncated variant (amino acids 761 to 1619) of GtfB of Lactobacillus reuteri strain 121 lacking both the N-terminal variable domain and domain V Limosilactobacillus reuteri

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri A0A0U5F702
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Limosilactobacillus reuteri 121 A0A0U5F702
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme mutant GtfB-DELTAN-DELTAV from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose V + H2O hydrolytic activity, amylose V with Mw 170 kDa Limosilactobacillus reuteri ?
-
 ?
amylose V + H2O hydrolytic activity, amylose V with Mw 170 kDa Limosilactobacillus reuteri 121 ?
-
 ?
amylose V + maltose transglycosylation activity, amylose V with 170 kDa Limosilactobacillus reuteri ?
-
 ?
amylose V + maltose transglycosylation activity, amylose V with 170 kDa Limosilactobacillus reuteri 121 ?
-
 ?
additional information the enzyme performs transglycosylation and/or hydrolytic activity. The linear alpha(1->4)-linked glucose units disappear and linear alpha(1->6)-linked glucose units appear. The total enzyme activity of GtfB-DELTAN-DELTAV is determined by the amylose-iodine staining method. Synthesis of isomalto/malto-polysaccharides (IMMP) from starch. Reaction product analyses, the IMMP product consists of terminal, and 6-substituted glucosyl units, overview. IMMP with 18.3 kDa Limosilactobacillus reuteri ?
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-
additional information the enzyme performs transglycosylation and/or hydrolytic activity. The linear alpha(1->4)-linked glucose units disappear and linear alpha(1->6)-linked glucose units appear. The total enzyme activity of GtfB-DELTAN-DELTAV is determined by the amylose-iodine staining method. Synthesis of isomalto/malto-polysaccharides (IMMP) from starch. Reaction product analyses, the IMMP product consists of terminal, and 6-substituted glucosyl units, overview. IMMP with 18.3 kDa Limosilactobacillus reuteri 121 ?
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-

Synonyms

Synonyms Comment Organism
GtfB
-
 Limosilactobacillus reuteri
UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase stabilizing protein UniProt Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40 assay at Limosilactobacillus reuteri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 purified recombinant enzyme, 20 min, inactivation Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Limosilactobacillus reuteri

General Information

General Information Comment Organism
physiological function identification of 4,6-alpha-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave alpha(1->4)-linkages in amylose and introduce alpha(1->6)-linkages in linear chains. The 4,6-alpha-glucanotransferase of Lactobacillus reuteri strain 121 converts amylose into an isomalto/malto-polysaccharide (IMMP) with 90% alpha(1->6)-linkages Limosilactobacillus reuteri