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Literature summary for 2.4.1.B34 extracted from
- Biochemical characterization of two GH70 family 4,6-alpha-glucanotransferases with distinct product specificity from Lactobacillus aviarius subsp. aviarius DSM 20655 (2018), Food Chem., 253, 236-246 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gtfX, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Ligilactobacillus aviarius |
| gene gtfY, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Ligilactobacillus aviarius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of deletion mutant GtfX-DELTANDELTAC encoding residues 448-1304, determination of the mutant product specificity, 1HNMR analysis is performed with the product mixtures obtained from MOS DP3-7, amylose V, starch, and amylopectin | Ligilactobacillus aviarius |
| additional information | construction of deletion mutant GtfY-DELTANDELTAC encoding residues 438-1290, determination of the mutant product specificity, 1HNMR analysis is performed with the product mixtures obtained from MOS DP3-7, amylose V, starch, and amylopectin | Ligilactobacillus aviarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ligilactobacillus aviarius | - |
- |
- |
| Ligilactobacillus aviarius DSM 20655 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Ligilactobacillus aviarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.9 | - |
purified recombinant enzyme mutant GtfX-DELTANDELTAC, pH 4.0, 53°C, substrate 0.125% w/v amylose V | Ligilactobacillus aviarius |
| 19.6 | - |
purified recombinant enzyme mutant GtfY-DELTANDELTAC, pH 5.0, 62°C, substrate 0.125% w/v amylose V | Ligilactobacillus aviarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + acceptor | transglycosylation activity | Ligilactobacillus aviarius | ? | - |
? |  |
| amylopectin + H2O | hydrolytic activity | Ligilactobacillus aviarius | ? | - |
? | |
| amylopectin + H2O | hydrolytic activity | Ligilactobacillus aviarius DSM 20655 | ? | - |
? | |
| amylose V + acceptor | transglycosylation activity | Ligilactobacillus aviarius | ? | - |
? | |
| amylose V + H2O | hydrolytic activity | Ligilactobacillus aviarius | ? | - |
? | |
| amylose V + H2O | hydrolytic activity | Ligilactobacillus aviarius DSM 20655 | ? | - |
? | |
| additional information | mutant GtfX-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfX-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfX-DELTANDELTAC from amylose V, overview | Ligilactobacillus aviarius | ? | - |
- |
|
| additional information | mutant GtfY-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfY-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfY-DELTANDELTAC from amylose V, overview | Ligilactobacillus aviarius | ? | - |
- |
|
| additional information | mutant GtfX-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfX-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfX-DELTANDELTAC from amylose V, overview | Ligilactobacillus aviarius DSM 20655 | ? | - |
- |
|
| additional information | mutant GtfY-DELTANDELTAC is inactive on sucrose, panose, nigerose, isomaltose, isomaltotriose, maltose, maltotriose, alpha-cyclodextrin, pullulan and dextran, but shows clear hydrolase/transglycosylase activity on malto-oligosaccharides (MOS) of DP4-7, amylose V, starch, and amylopectin. Substrate specificity of mutant GtfY-DELTANDELTAC, 1HNMR analysis product analysis, and structural analysis of polysaccharides generated by GtfY-DELTANDELTAC from amylose V, overview | Ligilactobacillus aviarius DSM 20655 | ? | - |
- |
|
| starch + acceptor | transglycosylation activity | Ligilactobacillus aviarius | ? | - |
? | |
| starch + H2O | hydrolytic activity | Ligilactobacillus aviarius | ? | - |
? | |
| starch + H2O | hydrolytic activity | Ligilactobacillus aviarius DSM 20655 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 100000, about, recombinant enzyme mutant GtfX-DELTANDELTAC, SDS-PAGE | Ligilactobacillus aviarius |
| ? | x * 100000, about, recombinant enzyme mutant GtfY-DELTANDELTAC, SDS-PAGE | Ligilactobacillus aviarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4,6-alpha-GT | - |
Ligilactobacillus aviarius |
| GtfB-like 4,6-alpha-glucanotransferases | - |
Ligilactobacillus aviarius |
| GtfX | - |
Ligilactobacillus aviarius |
| GtfY | - |
Ligilactobacillus aviarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
enzyme mutant GtfX-DELTANDELTAC | Ligilactobacillus aviarius |
| 62 | - |
enzyme mutant GtfY-DELTANDELTAC | Ligilactobacillus aviarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | enzyme mutant GtfX-DELTANDELTAC shows more than 60% of maximal activity within this temperature range | Ligilactobacillus aviarius |
| 58 | 73 | enzyme mutant GtfY-DELTANDELTAC shows more than 90% of maximal activity within this temperature range | Ligilactobacillus aviarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
enzyme mutant GtfX-DELTANDELTAC | Ligilactobacillus aviarius |
| 5 | - |
enzyme mutant GtfY-DELTANDELTAC | Ligilactobacillus aviarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 5.5 | enzyme mutant GtfY-DELTANDELTAC shows more than 60% of maximal activity within this pH range | Ligilactobacillus aviarius |
| 3 | 7 | enzyme mutant GtfX-DELTANDELTAC shows more than 60% of maximal activity within this pH range | Ligilactobacillus aviarius |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | structural modeling of Lactobacillus aviarius subsp. aviarius GtfX, compared to the crystal structure of Lactobacillus reuteri GtfB | Ligilactobacillus aviarius |
| additional information | structural modeling of Lactobacillus aviarius subsp. aviarius GtfY, compared to the crystal structure of Lactobacillus reuteri GtfB | Ligilactobacillus aviarius |
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Release 2023.1 (January 2023)
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