Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.B34 extracted from

  • Leemhuis, H.; Dijkman, W.; Dobruchowska, J.; Pijning, T.; Grijpstra, P.; Kralj, S.; Kamerling, J.; Dijkhuizen, L.
    4,6-alpha-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily (2013), Appl. Microbiol. Biotechnol., 97, 181-193.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.B34

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally truncated variant, in Escherichia coli Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
150
-
 maltose relaese of glucose, pH 4.7, 37°C Limosilactobacillus reuteri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
154000
-
 x * 154000, calculated Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri A5VL73
-
-
Limosilactobacillus reuteri Q5SBN1
-
-
Limosilactobacillus reuteri DSM 20016 A5VL73
-
-
Limosilactobacillus reuteri ML1 Q5SBN1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Maltoheptaose
-
 Limosilactobacillus reuteri ?
-
 ?
Maltoheptaose
-
 Limosilactobacillus reuteri ML1 ?
-
 ?
Maltoheptaose
-
 Limosilactobacillus reuteri DSM 20016 ?
-
 ?
Maltohexaose
-
 Limosilactobacillus reuteri ?
-
 ?
Maltohexaose
-
 Limosilactobacillus reuteri ML1 ?
-
 ?
Maltohexaose
-
 Limosilactobacillus reuteri DSM 20016 ?
-
 ?
maltopentaose
-
 Limosilactobacillus reuteri ?
-
 ?
maltopentaose
-
 Limosilactobacillus reuteri ML1 ?
-
 ?
maltopentaose
-
 Limosilactobacillus reuteri DSM 20016 ?
-
 ?
maltose
-
 Limosilactobacillus reuteri ?
-
 ?
maltotetraose + H2O
-
 Limosilactobacillus reuteri D-glucose + maltotetraose first clear products of the reaction. The enzyme is rather hydrolytic at the start of the reaction. When reaction products start to accumulate, transglycosylation becomes more efficient ?
maltotetraose + H2O
-
 Limosilactobacillus reuteri ML1 D-glucose + maltotetraose first clear products of the reaction. The enzyme is rather hydrolytic at the start of the reaction. When reaction products start to accumulate, transglycosylation becomes more efficient ?
maltotriose
-
 Limosilactobacillus reuteri ?
-
 ?
maltotriose
-
 Limosilactobacillus reuteri DSM 20016 ?
-
 ?
additional information enzyme cleaves alpha1->4 glycosidic linkages and adds the released glucose moieties one by one to the non-reducing end of growing linear alpha-glucan chains via alpha1->6 glycosidic linkages (alpha1->4 to alpha1->6 transfer activity). It converts pure maltooligosaccharide substrates into linear alpha-glucan product mixtures with about 50% alpha1->6 glycosidic bonds (isomalto/maltooligosaccharides). Largest products synthesized from maltoheptaose have a degree of polymerizatition bleow 50 Limosilactobacillus reuteri ?
-
 ?
additional information enzyme cleaves alpha1->4 glycosidic linkages and adds the released glucose moieties one by one to the non-reducing end of growing linear alpha-glucan chains via alpha1->6 glycosidic linkages (alpha1->4 to alpha1->6 transfer activity). It converts pure maltooligosaccharide substrates into linear alpha-glucan product mixtures with about 50% alpha1->6 glycosidic bonds (isomalto/maltooligosaccharides). Largest products synthesized from maltoheptaose have a degree of polymerizatition bleow 50 Limosilactobacillus reuteri ML1 ?
-
 ?
additional information enzyme cleaves alpha1->4 glycosidic linkages and adds the released glucose moieties one by one to the non-reducing end of growing linear alpha-glucan chains via alpha1->6 glycosidic linkages (alpha1->4 to alpha1->6 transfer activity). It converts pure maltooligosaccharide substrates into linear alpha-glucan product mixtures with about 50% alpha1->6 glycosidic bonds (isomalto/maltooligosaccharides). Largest products synthesized from maltoheptaose have a degree of polymerizatition bleow 50 Limosilactobacillus reuteri DSM 20016 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 154000, calculated Limosilactobacillus reuteri

Synonyms

Synonyms Comment Organism
GflML4
-
 Limosilactobacillus reuteri
GtfW
-
 Limosilactobacillus reuteri
Lreu_1346
-
 Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Limosilactobacillus reuteri
40 45
-
 Limosilactobacillus reuteri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37
-
 maltose relaese of glucose, pH 4.7, 37°C Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Limosilactobacillus reuteri
4.7
-
 assay at Limosilactobacillus reuteri