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Literature summary for 2.4.1.99 extracted from
- Fructooligosaccharides production by Schedonorus arundinaceus sucrose sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris (2018), J. Biotechnol., 266, 59-71 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the enzyme fused to the Saccharomyces cerevisiae alpha-factor signal peptide and a C-terminal His6 tag is expressed in Pichia pastoris strain X-33 | Lolium arundinaceum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a concentration of 10 mM is deleterious for enzyme activity | Lolium arundinaceum |  |
| EDTA | 4.7% inhibition at 1 mM | Lolium arundinaceum | |
| Fe2+ | a concentration of 10 mM is deleterious for enzyme activity | Lolium arundinaceum | |
| Hg2+ | a concentration of 10 mM is deleterious for enzyme activity | Lolium arundinaceum | |
| Ni2+ | a concentration of 10 mM is deleterious for enzyme activity | Lolium arundinaceum | |
| Zn2+ | a concentration of 10 mM is deleterious for enzyme activity | Lolium arundinaceum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the addition of NaCl, KCl, CaCl2 MnCl2, CoCl2 or MgCl2 does not vary the initial rates of glucose released from sucrose | Lolium arundinaceum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lolium arundinaceum | - |
tall fescue | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosylation influences the enzyme stability. The removal of N-linked oligosaccharides by Endo Hf treatment decreases the enzyme stability | Lolium arundinaceum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q sepharose column chromatography | Lolium arundinaceum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose + 1-kestose | 23% activity compared to sucrose | Lolium arundinaceum | fructose + nystose | - |
? | |
| crystalline cane sugar | - |
Lolium arundinaceum | 1-kestose + sucrose + D-glucose + nystose | - |
? | |
| fructosyl-nystose + H2O | 4% activity compared to sucrose. The reaction generates mainly hydrolytic products | Lolium arundinaceum | ? | - |
? | |
| additional information | no activity with melezitose, inulin and levan | Lolium arundinaceum | ? | - |
- |
|
| nystose + H2O | 8% activity compared to sucrose. The reaction generates mainly hydrolytic products | Lolium arundinaceum | ? | - |
? | |
| raffinose + H2O | 14% activity compared to sucrose. The reaction generates mainly hydrolytic products | Lolium arundinaceum | ? | - |
? | |
| stachyose + H2O | 2% activity compared to sucrose. The reaction generates mainly hydrolytic products | Lolium arundinaceum | ? | - |
? | |
| sucrose + sucrose | 100% activity | Lolium arundinaceum | 1-kestose + nystose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000, SDS-PAGE | Lolium arundinaceum |
| monomer | 1 * 64100, calculated from amino acid sequence | Lolium arundinaceum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-SST | - |
Lolium arundinaceum |
| sucrose-sucrose 1-fructosyltransferase | - |
Lolium arundinaceum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Lolium arundinaceum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | the enzyme shows more than 70% activity between 30 and 55°C. 40 and 7% of the optimum activity is measured at 60 and 70°C, respectively | Lolium arundinaceum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | the half-life values of the enzyme incubated at 30, 40 and 50°C are more than 7 days, 74 h and 16 h, respectively. Temperatures higher than 55°C inactivated the enzyme. In the absence of methyl-alpha-D-glucopyranoside, the enzyme behaves significantly less thermostable (half-life of 42 min at 40°C in 100 mM sodium acetate buffer, pH 5.5) | Lolium arundinaceum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Lolium arundinaceum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | the enzyme is active at pH 5.0-6.0. The enzyme shows more than 70% activity between pH 4.0 and 8.0 | Lolium arundinaceum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | between pH 5.0 and 6.5 (at 30°C), the enzyme shows remaining activities above 95% after 24 h incubation | Lolium arundinaceum |
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