Literature summary for 2.4.1.97 extracted from

Kuhaudomlarp, S.; Patron, N.; Henrissat, B.; Rejzek, M.; Saalbach, G.; Field, R.
Identification of Euglena gracilis beta-1,3-glucan phosphorylase and establishment of a new glycoside hydrolase (GH) family GH149 (2018), J. Biol. Chem., 293, 2865-2876 .

Search for more literature for 2.4.1.97

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Euglena gracilis var. saccharophila

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxynojirimycin	weak competitive inhibition	Euglena gracilis var. saccharophila

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.56	-	alpha-D-glucose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
0.67	-	laminaribiose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.26	-	laminaritriose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.41	-	laminaritetraose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
2.29	-	Laminaripentaose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
2.88	-	Laminarihexaose	pH 7.0, 30°C	Euglena gracilis var. saccharophila

Organism

Organism	UniProt	Comment	Textmining
Euglena gracilis var. saccharophila	A0A2I6SUQ9	glycoside hydrolase family GH149	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-glucose + glucose 1-phosphate	-	Euglena gracilis var. saccharophila	phosphate + laminaribiose	-	r
laminaribiose + glucose 1-phosphate	-	Euglena gracilis var. saccharophila	phosphate + laminaritriose	-	r
laminarihexaose + alpha-D-glucose 1-phosphate	-	Euglena gracilis var. saccharophila	laminariheptaose + phosphate	-	r
laminaripentaose + alpha-D-glucose 1-phosphate	-	Euglena gracilis var. saccharophila	laminarihexaose + phosphate	-	r
laminaritetraose + alpha-D-glucose 1-phosphate	-	Euglena gracilis var. saccharophila	laminaripentaose + phosphate	-	r
laminaritriose + alpha-D-glucose 1-phosphate	-	Euglena gracilis var. saccharophila	laminaritetraose + phosphate	-	r
laminaritriose + phosphate	-	Euglena gracilis var. saccharophila	laminaribiose + alpha-D-glucose 1-phosphate	-	?
additional information	protein is able to catalyze the conversion of glucose and glucose 1-phosphate into oligosaccharides with degree of polymerization up to 12 glucose units. Tps3 acts exclusively on glucose-beta-1,3-glucose oligosaccharides.The activity is dependent on both glucose and glucose 1-phosphate as substrates, excluding either from the reaction mixture completely abolishes oligosaccharide production	Euglena gracilis var. saccharophila	?	-	-

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	40	-	Euglena gracilis var. saccharophila

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.08	-	laminaribiose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.1	-	alpha-D-glucose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.1	-	Laminarihexaose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.12	-	laminaritriose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.12	-	laminaritetraose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.13	-	Laminaripentaose	pH 7.0, 30°C	Euglena gracilis var. saccharophila

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	-	Euglena gracilis var. saccharophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.38	-	Laminarihexaose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
0.5	-	Laminaripentaose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
0.79	-	laminaritetraose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
0.89	-	laminaritriose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.62	-	laminaribiose	pH 7.0, 30°C	Euglena gracilis var. saccharophila
1.99	-	alpha-D-glucose	pH 7.0, 30°C	Euglena gracilis var. saccharophila

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Release 2023.1 (January 2023)