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Literature summary for 2.4.1.97 extracted from
- Purification, crystallization and properties of a beta-(1->3)-glucan phosphorylase from Ochromonas malhamensis (1971), Phytochemistry, 10, 1293-1298.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Ochromonas malhamensis |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing, loss of activity | Ochromonas malhamensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | non-competitive inhibition at high concentration | Ochromonas malhamensis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
Laminarin | - |
Ochromonas malhamensis | |
| 12 | - |
glucose 1-phosphate | - |
Ochromonas malhamensis | |
| 25 | - |
phosphate | - |
Ochromonas malhamensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ochromonas malhamensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ochromonas malhamensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.92 | - |
- |
Ochromonas malhamensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, stable for several weeks | Ochromonas malhamensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,3-beta-D-glucosyl)n + phosphate | - |
Ochromonas malhamensis | (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
| laminaribiose + glucose 1-phosphate | - |
Ochromonas malhamensis | phosphate + laminarin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22.5 | - |
- |
Ochromonas malhamensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 16 | 27 | about 50% of activity maximum at 16°C and 27°C | Ochromonas malhamensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Ochromonas malhamensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | pH 5.0: 50% of activity maximum, pH 5.5: activity maximum | Ochromonas malhamensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| AMP | low concentration: stimulation, Km: 0.04 mM | Ochromonas malhamensis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
AMP | - |
Ochromonas malhamensis | |
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