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Literature summary for 2.4.1.97 extracted from

  • Albrecht, G.J.; Kauss, H.
    Purification, crystallization and properties of a beta-(1->3)-glucan phosphorylase from Ochromonas malhamensis (1971), Phytochemistry, 10, 1293-1298.
No PubMed abstract available
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Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Poterioochromonas malhamensis

General Stability

General Stability Organism
freezing and thawing, loss of activity Poterioochromonas malhamensis

Inhibitors

Inhibitors Comment Organism Structure
AMP non-competitive inhibition at high concentration Poterioochromonas malhamensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 Laminarin
-
 Poterioochromonas malhamensis
12
-
 glucose 1-phosphate
-
 Poterioochromonas malhamensis
25
-
 phosphate
-
 Poterioochromonas malhamensis

Organism

Organism UniProt Comment Textmining
Poterioochromonas malhamensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Poterioochromonas malhamensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.92
-
-
 Poterioochromonas malhamensis

Storage Stability

Storage Stability Organism
0-4°C, stable for several weeks Poterioochromonas malhamensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,3-beta-D-glucosyl)n + phosphate
-
 Poterioochromonas malhamensis (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 ?
laminaribiose + glucose 1-phosphate
-
 Poterioochromonas malhamensis phosphate + laminarin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22.5
-
-
 Poterioochromonas malhamensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
16 27 about 50% of activity maximum at 16°C and 27°C Poterioochromonas malhamensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Poterioochromonas malhamensis

pH Range

pH Minimum pH Maximum Comment Organism
5 5.5 pH 5.0: 50% of activity maximum, pH 5.5: activity maximum Poterioochromonas malhamensis

Cofactor

Cofactor Comment Organism Structure
AMP low concentration: stimulation, Km: 0.04 mM Poterioochromonas malhamensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
20
-
 AMP
-
 Poterioochromonas malhamensis