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Literature summary for 2.4.1.91 extracted from

  • Kovinich, N.; Saleem, A.; Arnason, J.T.; Miki, B.
    Functional characterization of a UDP-glucose:flavonoid 3-O-beta-D-glucosyltransferase from the seed coat of black soybean (Glycine max (L.) Merr.) (2010), Phytochemistry, 71, 1253-1263.
    View publication on PubMed
Search for more literature for 2.4.1.91

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells and in a Arabidopsis thaliana mutant (ugt78d2) deficient in anthocyanidin and flavonol 3-O-glucosyltransferase activity Glycine max

Inhibitors

Inhibitors Comment Organism Structure
cyanidin the recombinant enzyme exhibits pronounced substrate inhibition by cyanidin at 0.1 mM Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.174
-
 kaempferol recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30°C Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49240
-
 x * 49240, calculated from amino acid sequence Glycine max
49800
-
 x * 49800, estimated from SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max D3Y5N8 cf. EC 2.4.1.115; cultivar Clark line
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity column chromatography Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Glycine max
-
flower bud
-
 Glycine max
-
leaf
-
 Glycine max
-
pod
-
 Glycine max
-
root
-
 Glycine max
-
seed coat
-
 Glycine max
-
stem
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with dihydroquercetin, genistin, genistein, daidzein, catechin, epicatechin, apigeninidin, flavonol 3-O-glucosides, anthocyanidin 3-O-glucosides and cyanidin 3-O-galactoside Glycine max ?
-
 ?
UDP-alpha-D-glucose + cyanidin
-
 Glycine max UDP + cyanidin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin best substrate Glycine max UDP + delphinidin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + isorhamnetin
-
 Glycine max UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + kaempferol
-
 Glycine max UDP + kaempferol 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + malvidin
-
 Glycine max UDP + malvidin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + myricetin
-
 Glycine max UDP + myricetin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin
-
 Glycine max UDP + pelargonidin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + peonidin
-
 Glycine max UDP + peonidin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + petunidin
-
 Glycine max UDP + petunidin 3-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + quercetin
-
 Glycine max UDP + quercetin 3-O-beta-D-glucoside
-
 ?
UDP-galactose + cyanidin galactose can be transferred from UDP-galactose to the 3-position of cyanidin with low specificity relative to the analogous glucosyltransferase reactions Glycine max ?
-
 ?
UDP-galactose + delphinidin galactose can be transferred from UDP-galactose to the 3-position of delphinidin with low specificity relative to the analogous glucosyltransferase reactions Glycine max ?
-
 ?

Subunits

Subunits Comment Organism
? x * 49240, calculated from amino acid sequence Glycine max
? x * 49800, estimated from SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
UDP-glucose:flavonoid 3-O-glucosyltransferase
-
 Glycine max
UGT78K1
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.25
-
 kaempferol recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30°C Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 the recombinant enzyme exhibits a slight buffer preference for HEPES over Tris at pH 8.0 Glycine max

Expression

Organism Comment Expression
Glycine max the enzyme is expressed not only at the sites of anthocyanin biosynthesis, but also at sites of flavonol glycoside biosynthesis up

General Information

General Information Comment Organism
physiological function the enzyme functions as a 3-O-glucosyltransferase in anthocyanin and flavonol biosynthesis in vivo Glycine max