Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-lactalbumin | enzyme binds to the effector | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and mutant W314A in Escherichia coli in inclusion bodies | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
catalytic enzyme domain without complexed substrate, conf-I' conformation, X-ray diffraction structure determination and analysis at 1.9 A resolution | Bos taurus |
W314A enzyme mutant complexed with alpha-lactalbumin in presence of N-acetylglucoamine, UDP, and Mn2+, conf-II conformation, X-ray diffraction structure determination and analysis at 2.3 A resolution | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
W314A | site-directed mutagenesis, mutant shows highly reduced activity, i.e. 0.6% of wild-type galactosyltransferase activity, substrate binding, and reduced binding to the effector alpha-lactalbumin as well as reduced susceptibility to cleavage by proteases Glu-C and Lys-C compared to the wild-type enzyme, overview | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Mus musculus | 5794 | - |
Golgi apparatus | - |
Bos taurus | 5794 | - |
membrane | - |
Mus musculus | 16020 | - |
membrane | - |
Bos taurus | 16020 | - |
additional information | enzyme possesses a membrane-spanning domain, a cytoplasmic domain, a stem region, and the catalytic domain that faces the lumen | Mus musculus | - |
- |
additional information | enzyme possesses a membrane-spanning domain, a cytoplasmic domain, a stem region, and the catalytic domain that faces the lumen | Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required by full length wild-type enzyme | Bos taurus | |
Mn2+ | required by full length wild-type enzyme, coordination structure | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + N-acetyl-D-glucosamine | Mus musculus | - |
UDP + N-acetyllactosamine | - |
? | |
UDP-galactose + N-acetyl-D-glucosamine | Bos taurus | - |
UDP + N-acetyllactosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and mutant W314A from Escherichia coli after solubilization from inclusion bodies by alpha-lactalbumin affinity chromatography | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine | catalytic mechanism, enzyme undergoes critical conformational changes upon substrate binding from an open, conf-I, to a closed conformation, conf-II, involving 2 flexible loops, a small and a long one, with Trp314 of the small loop being critical for the change interacting with both substrates, catalytic site structure | Bos taurus | |
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine | catalytic mechanism, enzyme undergoes critical conformational changes upon substrate binding from an open, conf-I, to a closed conformation, conf-II, involving 2 flexible loops, a small and a long one, with Trp314 of the small loop being critical for the change interacting with both substrates, catalytic site structure, substrate binding structure | Mus musculus |
Renatured (Comment) | Organism |
---|---|
refolding of the wild-type enzyme and mutant W314A from inclusion bodies after recombinant expression in Escherichia coli | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + N-acetyl-D-glucosamine | - |
Mus musculus | UDP + N-acetyllactosamine | - |
? | |
UDP-galactose + N-acetyl-D-glucosamine | - |
Bos taurus | UDP + N-acetyllactosamine | - |
? | |
UDP-galactose + N-acetyl-D-glucosamine | substrate binding structure | Mus musculus | UDP + N-acetyllactosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme possesses a membrane-spanning domain, a cytoplasmic domain, a stem region, and the catalytic domain that faces the lumen | Mus musculus |
More | enzyme possesses a membrane-spanning domain, a cytoplasmic domain, a stem region, and the catalytic domain that faces the lumen | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase-I | - |
Mus musculus |
beta1,4-galactosyltransferase-I | - |
Bos taurus |
beta4Gal-T1 | - |
Mus musculus |
beta4Gal-T1 | - |
Bos taurus |