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Literature summary for 2.4.1.9 extracted from

  • Charoenwongpaiboon, T.; Wangpaiboon, K.; Pichyangkura, R.; Prousoontorn, M.
    Highly porous core-shell chitosan beads with superb immobilization efficiency for Lactobacillus reuteri 121 inulosucrase and production of inulin-type fructooligosaccharides (2018), RSC Adv., 8, 17008-17016 .
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Limosilactobacillus reuteri

General Stability

General Stability Organism
enzyme immobilized on core-shell chitosan beads retains approximately 45% of its initial catalytic activity after being reused for 12 cycles Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(2->1)-beta-D-fructosyl]n Limosilactobacillus reuteri
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Limosilactobacillus reuteri 121
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri Q8GP32
-
-
Limosilactobacillus reuteri 121 Q8GP32
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by Toyopearl DEAE-650M column chromatography Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose
-
 Limosilactobacillus reuteri alpha-D-glucose + inulin-type fructooligosaccharides
-
 ?
sucrose + sucrose
-
 Limosilactobacillus reuteri 121 alpha-D-glucose + inulin-type fructooligosaccharides
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Limosilactobacillus reuteri alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Limosilactobacillus reuteri 121 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 87000, SDS-PAGE Limosilactobacillus reuteri

Synonyms

Synonyms Comment Organism
INU
-
 Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 free enzyme Limosilactobacillus reuteri
60
-
 enzyme immobilized on core-shell chitosan beads Limosilactobacillus reuteri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 the free enzyme shows more than 50% activity between 40 and 60°C. The enzyme is inactive at 70°C Limosilactobacillus reuteri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the immobilized enzyme retains about 84% of activity after incubation at 50°C for 12 h, whereas the residual activity of the free enzyme is lower than 50% under the same conditions Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 free enzyme and enzyme immobilized on core-shell chitosan beads Limosilactobacillus reuteri

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10 the enzyme remains stable after 3 h at pH 2.0-10.0. The free and immobilized enzymes retain 80 and 100% activity after 3 h at pH 4.0-8.0, respectively Limosilactobacillus reuteri