Literature summary for 2.4.1.9 extracted from

Ko, H.; Bae, J.H.; Sung, B.H.; Kim, M.J.; Park, H.J.; Sohn, J.H.
Microbial production of medium chain fructooligosaccharides by recombinant yeast secreting bacterial inulosucrase (2019), Enzyme Microb. Technol., 130, 109364 .

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Cloned(Commentary)

Cloned (Comment)	Organism
the N- and C-terminally truncated enzyme is expressed in Saccharomyces cerevisiae strain 2805gs	Limosilactobacillus reuteri

Organism

Organism	UniProt	Comment	Textmining
Limosilactobacillus reuteri	Q8GP32	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + sucrose	with 300 g/l sucrose, 128.4 g/l of fructooligosaccharide is produced with 85.6% conversion yield. From 400 g/l sucrose, 152.6 g/l of fructooligosaccharides is produced with 76.3% conversion yield	Limosilactobacillus reuteri	alpha-D-glucose + medium chain fructooligosaccharides (DP 2-20)	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, N- and C-terminally truncated enzyme, SDS-PAGE	Limosilactobacillus reuteri
?	x * 83000, mature enzyme, SDS-PAGE	Limosilactobacillus reuteri

Synonyms

Synonyms	Comment	Organism
INU	-	Limosilactobacillus reuteri

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Release 2023.1 (January 2023)