Cloned (Comment) | Organism |
---|---|
recombinant overexpression of the enzyme lacking the cell-anchoring motif in Escherichia coli | Lactobacillus gasseri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | establishing of an enzymatic synthesis process of a series of raffinose-derived oligosaccharides or raffinosyl oligofructosides (RFOS) with degree of polymerization from 4 to 8 developed in presence of raffinose and involving a transfructosylation reaction catalyzed by an inulosucrase from Lactobacillus gasseri DSM 20604, method development, overview. The main synthesized RFOS consist of beta-2,1-linked fructose unit(s) to raffinose: alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1<->2)-beta-D-fructofuranosyl-((1<-2)-beta-D-fructofuranoside)n (where n refers to the number of transferred fructose moieties), structure analysis by NMR spectroscopy | Lactobacillus gasseri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus gasseri | - |
- |
- |
Lactobacillus gasseri DSM 20604 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside | Lactobacillus gasseri | ? | - |
? | |
additional information | sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside | Lactobacillus gasseri DSM 20604 | ? | - |
? | |
raffinose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus gasseri | glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
raffinose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus gasseri DSM 20604 | glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
sucrose + melibiose | - |
Lactobacillus gasseri | glucose + ? | - |
? | |
sucrose + melibiose | - |
Lactobacillus gasseri DSM 20604 | glucose + ? | - |
? | |
sucrose + raffinose | - |
Lactobacillus gasseri | glucose + 1-kestose | i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside | ? | |
sucrose + raffinose | - |
Lactobacillus gasseri DSM 20604 | glucose + 1-kestose | i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside | ? | |
sucrose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus gasseri | glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
sucrose + [(2->1)-beta-D-fructosyl]n | - |
Lactobacillus gasseri DSM 20604 | glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Lactobacillus gasseri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
assay at | Lactobacillus gasseri |