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Literature summary for 2.4.1.9 extracted from

  • Diez-Municio, M.; Herrero, M.; de Las Rivas, B.; Munoz, R.; Jimeno, M.L.; Moreno, F.J.
    Synthesis and structural characterization of raffinosyl-oligofructosides upon transfructosylation by Lactobacillus gasseri DSM 20604 inulosucrase (2016), Appl. Microbiol. Biotechnol., 100, 6251-6263.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of the enzyme lacking the cell-anchoring motif in Escherichia coli Lactobacillus gasseri

Protein Variants

Protein Variants Comment Organism
additional information establishing of an enzymatic synthesis process of a series of raffinose-derived oligosaccharides or raffinosyl oligofructosides (RFOS) with degree of polymerization from 4 to 8 developed in presence of raffinose and involving a transfructosylation reaction catalyzed by an inulosucrase from Lactobacillus gasseri DSM 20604, method development, overview. The main synthesized RFOS consist of beta-2,1-linked fructose unit(s) to raffinose: alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1<->2)-beta-D-fructofuranosyl-((1<-2)-beta-D-fructofuranoside)n (where n refers to the number of transferred fructose moieties), structure analysis by NMR spectroscopy Lactobacillus gasseri

Organism

Organism UniProt Comment Textmining
Lactobacillus gasseri
-
-
-
Lactobacillus gasseri DSM 20604
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside Lactobacillus gasseri ?
-
?
additional information sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside Lactobacillus gasseri DSM 20604 ?
-
?
raffinose + [(2->1)-beta-D-fructosyl]n
-
Lactobacillus gasseri glucose + [(2->1)-beta-D-fructosyl]n+1
-
?
raffinose + [(2->1)-beta-D-fructosyl]n
-
Lactobacillus gasseri DSM 20604 glucose + [(2->1)-beta-D-fructosyl]n+1
-
?
sucrose + melibiose
-
Lactobacillus gasseri glucose + ?
-
?
sucrose + melibiose
-
Lactobacillus gasseri DSM 20604 glucose + ?
-
?
sucrose + raffinose
-
Lactobacillus gasseri glucose + 1-kestose i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside ?
sucrose + raffinose
-
Lactobacillus gasseri DSM 20604 glucose + 1-kestose i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
Lactobacillus gasseri glucose + [(2->1)-beta-D-fructosyl]n+1
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
-
Lactobacillus gasseri DSM 20604 glucose + [(2->1)-beta-D-fructosyl]n+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
assay at Lactobacillus gasseri