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Literature summary for 2.4.1.88 extracted from

  • Jacob, F.; Hitchins, M.P.; Fedier, A.; Brennan, K.; Nixdorf, S.; Hacker, N.F.; Ward, R.; Heinzelmann-Schwarz, V.A.
    Expression of GBGT1 is epigenetically regulated by DNA methylation in ovarian cancer cells (2014), BMC Mol. Biol., 15, 24.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene GBGT1, quantitative RT-PCR enzyme expression profiling, high or intermediate levels of GBGT1 DNA methylation in all ovarian cancer cell lines (except for OVCAR3) but marginal levels of DNA methylation in the two normal ovary surface epithelial cell lines Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens synthesis of Forssman (Fs) antigen, a pentaglycosyl ceramide (GalNAcalpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glcbeta1-1Cer) terminated by GalNAc and belongs to the globo series of glycosphingolipids ?
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N5D6
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Source Tissue

Source Tissue Comment Organism Textmining
A-2780 cell
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Homo sapiens
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HOSE 17-1 cell a normal ovarian surface epithelium cell line Homo sapiens
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HOSE 6-3 cell a normal ovarian surface epithelium cell line Homo sapiens
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additional information differential GBGT1 expression in various human tissue samples and disease stages Homo sapiens
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ovarian cancer cell primary or cell culture Homo sapiens
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ovary high enzyme expression level Homo sapiens
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OVCAR-3 cell
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Homo sapiens
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placenta high enzyme expression level Homo sapiens
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SKOV-3 cell
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Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information synthesis of Forssman (Fs) antigen, a pentaglycosyl ceramide (GalNAcalpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glcbeta1-1Cer) terminated by GalNAc and belongs to the globo series of glycosphingolipids Homo sapiens ?
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?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosylceramide
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Homo sapiens UDP + N-acetyl-D-galactosaminyl-(1->3)-N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosylceramide
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?

Synonyms

Synonyms Comment Organism
GBGT1
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Homo sapiens
globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
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Homo sapiens

Expression

Organism Comment Expression
Homo sapiens GBGT1 expression is epigenetically silenced through promoter hypermethylation in ovarian cancer. The extent of DNA methylation inversely correlates with GBGT1 mRNA and protein expression down
Homo sapiens restoration of GBGT1 mRNA and protein expression in low GBGT1-expressing A2780 cells is achieved by 5-aza-2'-deoxycytidine treatment additional information

General Information

General Information Comment Organism
malfunction GBGT1 expression is epigenetically silenced through promoter hypermethylation in ovarian cancer Homo sapiens
metabolism the enzyme catalyzes the last step in the multi-step biosynthesis of the Forssman antigen, Fs antigen, a pentaglycosyl ceramide of the globo series glycosphingolipids. Involvement of DNA methylation in the synthesis of Fs antigen Homo sapiens
additional information the extent of DNA methylation inversely correlates with GBGT1 mRNA and protein expression Homo sapiens