Cloned (Comment) | Organism |
---|---|
gene GBGT1, quantitative RT-PCR enzyme expression profiling, high or intermediate levels of GBGT1 DNA methylation in all ovarian cancer cell lines (except for OVCAR3) but marginal levels of DNA methylation in the two normal ovary surface epithelial cell lines | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | synthesis of Forssman (Fs) antigen, a pentaglycosyl ceramide (GalNAcalpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glcbeta1-1Cer) terminated by GalNAc and belongs to the globo series of glycosphingolipids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N5D6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-2780 cell | - |
Homo sapiens | - |
HOSE 17-1 cell | a normal ovarian surface epithelium cell line | Homo sapiens | - |
HOSE 6-3 cell | a normal ovarian surface epithelium cell line | Homo sapiens | - |
additional information | differential GBGT1 expression in various human tissue samples and disease stages | Homo sapiens | - |
ovarian cancer cell | primary or cell culture | Homo sapiens | - |
ovary | high enzyme expression level | Homo sapiens | - |
OVCAR-3 cell | - |
Homo sapiens | - |
placenta | high enzyme expression level | Homo sapiens | - |
SKOV-3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synthesis of Forssman (Fs) antigen, a pentaglycosyl ceramide (GalNAcalpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glcbeta1-1Cer) terminated by GalNAc and belongs to the globo series of glycosphingolipids | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosylceramide | - |
Homo sapiens | UDP + N-acetyl-D-galactosaminyl-(1->3)-N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosylceramide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GBGT1 | - |
Homo sapiens |
globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | GBGT1 expression is epigenetically silenced through promoter hypermethylation in ovarian cancer. The extent of DNA methylation inversely correlates with GBGT1 mRNA and protein expression | down |
Homo sapiens | restoration of GBGT1 mRNA and protein expression in low GBGT1-expressing A2780 cells is achieved by 5-aza-2'-deoxycytidine treatment | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | GBGT1 expression is epigenetically silenced through promoter hypermethylation in ovarian cancer | Homo sapiens |
metabolism | the enzyme catalyzes the last step in the multi-step biosynthesis of the Forssman antigen, Fs antigen, a pentaglycosyl ceramide of the globo series glycosphingolipids. Involvement of DNA methylation in the synthesis of Fs antigen | Homo sapiens |
additional information | the extent of DNA methylation inversely correlates with GBGT1 mRNA and protein expression | Homo sapiens |