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Literature summary for 2.4.1.88 extracted from

  • Xu, H.; Storch, T.; Yu, M.; Elliott, S.P.; Haslam, D.B.
    Characterization of the human Forssman synthetase gene. An evolving association between glycolipid synthesis and host-microbial interactions (1999), J. Biol. Chem., 274, 29390-29398.
    View publication on PubMed
Search for more literature for 2.4.1.88

Cloned(Commentary)

Cloned (Comment) Organism
cloned from cDNA library by PCR, expression, transcription and processing, of wild-type in COS-1 cells with and without HA-tag, but no detectable enzyme activity in the transfected cells by recombinant enzyme, DNA and amino acid sequence determination and analysis Homo sapiens
expression in COS-1 cells with and without HA-tag Canis lupus familiaris
expression of wild-type in T42 human bladder and Vero monkey kidney epithelial cells, enables the cells to bind a canine Escherichia coli uropathogen, which they cannot bind without transfection Canis lupus familiaris

Protein Variants

Protein Variants Comment Organism
additional information chimeric enzyme constructs of N-terminal canine sequence fused in frame with the C-terminal catalytic region of the human sequence, i.e. dog:human construct, and vice versa, i.e. human:dog construct, expression in COS-1 cells, activity is shown only by the human:dog construct Canis lupus familiaris
additional information chimeric enzyme constructs of N-terminal canine sequence fused in frame with the C-terminal catalytic region of the human sequence, i.e. dog:human construct, and vice versa, i.e. human:dog construct, expression in COS-1 cells, activity is shown only by the human:dog construct Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Canis lupus familiaris 16020
-
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence alignment and comparison, 83% identity Canis lupus familiaris
additional information
-
 amino acid sequence alignment and comparison, 83% identity Homo sapiens
additional information
-
 genomic organisation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens variability in glycolipid synthesis between species is an important determinant of microbial tropism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Homo sapiens
-
 cosmid sequence from chromosome 9q34
-
Homo sapiens Q8N5D6 immunoreactive sequence homologue to FS sequences, but without FS activity due to modification of catalytic domain during evolution
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme mRNA is detectable in all tissues Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information variability in glycolipid synthesis between species is an important determinant of microbial tropism Homo sapiens ?
-
 ?
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide no activity Homo sapiens UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 ?

Synonyms

Synonyms Comment Organism
Forssmann glycolipid synthetase
-
 Canis lupus familiaris
Forssmann glycolipid synthetase
-
 Homo sapiens
FS
-
 Canis lupus familiaris
FS
-
 Homo sapiens