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Literature summary for 2.4.1.87 extracted from

  • Chen, C.; Liu, B.; Xu, Y.; Utkina, N.; Zhou, D.; Danilov, L.; Torgov, V.; Veselovsky, V.; Feng, L.
    Biochemical characterization of the novel alpha-1, 3-galactosyltransferase WclR from Escherichia coli O3 (2016), Carbohydr. Res., 430, 36-43 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ assay in presence of 5 mM Mg2+, Mn2+, or Fe2+ Escherichia coli
Mg2+ assay in presence of 5 mM Mg2+, Mn2+, or Fe2+ Escherichia coli
Mn2+ assay in presence of 5 mM Mg2+, Mn2+, or Fe2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli B5T072
-
-
Escherichia coli O3 B5T072
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no donors: UDP-Glc, UDP-GlcNAc, UDPGalNAc, CMP-Neu and GDP-Man Escherichia coli ?
-
-
additional information no donors: UDP-Glc, UDP-GlcNAc, UDPGalNAc, CMP-Neu and GDP-Man Escherichia coli O3 ?
-
-
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
Escherichia coli UDP + alpha-D-galactosyl-(1->3)-N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
Escherichia coli O3 UDP + alpha-D-galactosyl-(1->3)-N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
?

Subunits

Subunits Comment Organism
? x * 70240, calculated from sequence, x * 66000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
wclR
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Escherichia coli