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Literature summary for 2.4.1.87 extracted from

  • Blanken, W.M.; van den Eijnden, D.H.
    Biosynthesis of terminal Gal alpha (1-3)Gal beta 1-4)GlcNAc-R oligosaccharide sequences on glycoconjugates. Purification and acceptor specificity of a UDP-Gal:N-acetyllactosaminide alpha(1-3)galactosyltransferase from calf thymus (1985), J. Biol. Chem., 260, 12927-12934.
    View publication on PubMed
Search for more literature for 2.4.1.87

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 Galbeta(1-4)GlcNAcbeta(1-2)Man(1-3)Manbeta(1-4)GlcNAc
-
 Bos taurus
1.25
-
 asialo-alpha1-acid glycoprotein
-
 Bos taurus
1.39
-
 beta-D-Gal(1-4)-D-GlcNAc
-
 Bos taurus
9
-
 beta-D-Gal(1-4)-D-Glc
-
 Bos taurus
16
-
 beta-D-Gal(1-3)beta-D-GlcNAc(1-3)beta-D-Gal(1-4)-D-Glc
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
calf Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acceptor substrate specificity Bos taurus ?
-
 ?
UDP-alpha-D-galactose + Galbeta(1-4)GlcNAcbeta(1-2)Man(1-3)Manbeta(1-4)GlcNAc
-
 Bos taurus UDP + alpha-D-galactosyl-1,3-Galbeta(1-4)GlcNAcbeta(1-2)Man(1-3)Manbeta(1-4)GlcNAc
-
 ?
UDP-galactose + asialo-alpha1-acid glycoprotein
-
 Bos taurus UDP + alpha-D-galactosyl-asialo-alpha1-acid glycoprotein
-
 ?
UDP-galactose + beta-D-Gal(1-3)beta-D-GlcNAc(1-3)beta-D-Gal(1-4)-D-Glc
-
 Bos taurus UDP + alpha-D-Gal(1-3)beta-D-Gal(1-3)beta-D-GlcNAc(1-3)beta-D-Gal(1-4)-D-Glc
-
 ?
UDP-galactose + beta-D-Gal(1-4)GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
 Bos taurus UDP + alpha-D-Gal(1-3)beta-D-Gal(1-4)GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
 ?
UDP-galactose + beta-D-galactosyl-1,4-D-glucose
-
 Bos taurus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-D-glucose
-
 ?
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 Bos taurus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 ?
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R highly active with glycoproteins, oligosaccharides and glycolipids having a terminal Galbeta(1-4)GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc and paragloboside Bos taurus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-galactose + glycoprotein
-
 Bos taurus UDP + alpha-D-galactosylglycoprotein
-
 ?

Subunits

Subunits Comment Organism
? x * 40000-42000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 charge heterogenity, pI varying between 5.0 and 6.5 Bos taurus