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Literature summary for 2.4.1.86 extracted from

  • Basu, M.; Basu, S.
    Enzymatic synthesis of a tetraglycosylceramide by a galactosyltransferase from rabbit bone marrow (1972), J. Biol. Chem., 247, 1489-1495.
    View publication on PubMed
Search for more literature for 2.4.1.86

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 UDPgalactose
-
 Oryctolagus cuniculus
0.5
-
 N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
-
 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 6-7% of the activity with Mn2+ Oryctolagus cuniculus
Co2+ 6-7% of the activity with Mn2+ Oryctolagus cuniculus
Mg2+ 6-7% of activity with Mn2+ Oryctolagus cuniculus
Mn2+ required Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDPglucose cannot substitute for UDPgalactose, Tay-Sachs ganglioside (GM2) is a poor galactose acceptor Oryctolagus cuniculus ?
-
 ?
UDPgalactose + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
-
 Oryctolagus cuniculus UDP + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Oryctolagus cuniculus