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Literature summary for 2.4.1.82 extracted from

  • Kito, K.; Yamane, K.; Yamamori, T.; Matsuhira, H.; Tanaka, Y.; Takabe, T.
    Isolation, functional characterization and stress responses of raffinose synthase genes in sugar beet (2018), J. Plant Biochem. Biotechnol., 27, 36-45 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16.1
-
sucrose pH 8.0, 45°C Beta vulgaris
28
-
galactinol pH 8.0, 45°C Beta vulgaris

Organism

Organism UniProt Comment Textmining
Beta vulgaris A0A1S7IYY0 isoform RS2
-
Beta vulgaris A0A1S7IYZ8 isoform RS1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Beta vulgaris
-
root
-
Beta vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactinol + sucrose
-
Beta vulgaris raffinose + myo-inositol
-
?

Subunits

Subunits Comment Organism
? x * 87094, calculated from sequence, x * 75000, SDS-PAGE of recombinant protein Beta vulgaris
? x * 87788, calculated from sequence, x * 75000, SDS-PAGE of recombinant protein Beta vulgaris

pI Value

Organism Comment pI Value Maximum pI Value
Beta vulgaris calculated from sequence
-
5.1
Beta vulgaris calculated from sequence
-
6.2

Expression

Organism Comment Expression
Beta vulgaris the expression levels of isoforms RS1 are significantly higher than that of isoform RS2 additional information
Beta vulgaris isoform RS2 is slowly induced by cold and salt stresses up
Beta vulgaris the mRNA for RS1 is rapidly induced by cold stress up