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Literature summary for 2.4.1.80 extracted from

  • Basu, S.; Kaufman, B.; Roseman, S.
    Enzymatic synthesis of glucocerebroside by a glucosyltransferase from embryonic chicken brain (1973), J. Biol. Chem., 248, 1388-1394.
    View publication on PubMed
Search for more literature for 2.4.1.80

Activating Compound

Activating Compound Comment Organism Structure
detergent
-
 Cavia porcellus
detergent
-
 Homo sapiens
detergent
-
 Rattus norvegicus
detergent
-
 Sus scrofa
detergent
-
 Ovis aries
detergent absolute requirement for Gallus gallus
detergent optimal activity obtained with a mixture of Cutsum and Triton X-100 (2:1) Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA Gallus gallus
additional information
-
 Homo sapiens
additional information
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 ceramide
-
 Gallus gallus
0.12
-
 UDP-glucose
-
 Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Gallus gallus 16020
-
membrane
-
 Rattus norvegicus 16020
-
membrane
-
 Sus scrofa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ not required Gallus gallus
Mn2+ not required Gallus gallus
additional information no stimulation by metal ions Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + N-acylsphingosine Gallus gallus first step in synthesis of gangliosides UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine Cavia porcellus first step in synthesis of gangliosides UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine Homo sapiens first step in synthesis of gangliosides UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine Rattus norvegicus first step in synthesis of gangliosides UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine Sus scrofa first step in synthesis of gangliosides UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine Ovis aries first step in synthesis of gangliosides UDP + D-glucosyl-N-acylsphingosine
-
 ?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Gallus gallus
-
 13-14 days old embryo
-
Homo sapiens
-
-
-
Ovis aries
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Gallus gallus
-
brain
-
 Cavia porcellus
-
brain
-
 Homo sapiens
-
brain
-
 Rattus norvegicus
-
brain
-
 Sus scrofa
-
brain
-
 Ovis aries
-
embryo
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity in brain homogenates Gallus gallus
additional information
-
 activity in brain homogenates Cavia porcellus
additional information
-
 activity in brain homogenates Homo sapiens
additional information
-
 activity in brain homogenates Rattus norvegicus
additional information
-
 activity in brain homogenates Sus scrofa
additional information
-
 activity in brain homogenates Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + dihydrosphingosine
-
 Gallus gallus UDP + D-glucosyl-dihydrosphingosine
-
 ?
UDP-glucose + N-acylsphingosine specific for UDP-glucose Gallus gallus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Gallus gallus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Cavia porcellus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Homo sapiens UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Sus scrofa UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine i.e. ceramide Ovis aries UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
UDP-glucose + N-acylsphingosine first step in synthesis of gangliosides Gallus gallus UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine first step in synthesis of gangliosides Cavia porcellus UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine first step in synthesis of gangliosides Homo sapiens UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine first step in synthesis of gangliosides Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine first step in synthesis of gangliosides Sus scrofa UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + N-acylsphingosine first step in synthesis of gangliosides Ovis aries UDP + D-glucosyl-N-acylsphingosine
-
 ?
UDP-glucose + sphingosine
-
 Gallus gallus UDP + D-glucosyl-sphingosine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gallus gallus
30
-
 assay at Cavia porcellus
30
-
 assay at Homo sapiens
30
-
 assay at Rattus norvegicus
30
-
 assay at Sus scrofa
30
-
 assay at Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Gallus gallus
7
-
 assay at Cavia porcellus
7
-
 assay at Homo sapiens
7
-
 assay at Rattus norvegicus
7
-
 assay at Sus scrofa
7
-
 assay at Ovis aries