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Literature summary for 2.4.1.71 extracted from

  • Frear, D.S.
    Herbicide Metabolism in plants-I. Purification and properties of UDP-glucose:arylamine N-glucosyltransferase from soybean (1968), Phytochemistry, 7, 381-390.
No PubMed abstract available

General Stability

General Stability Organism
ammonium sulfate at high concentrations inactivates Glycine max
freeze-thawing, stable to Glycine max
stable to centrifugation at 150000 rpm for 90 min Glycine max

Inhibitors

Inhibitors Comment Organism Structure
catechol weak Glycine max
CuSO4 strong Glycine max
GSSG oxidized glutathione, weak Glycine max
HgCl2 strong Glycine max
iodoacetamide weak Glycine max
additional information no inhibition by EDTA and iodoacetate, 1 mM each Glycine max
NEM
-
Glycine max
o-Iodosobenzoate
-
Glycine max
p-benzoquinone strong Glycine max
PCMB strong Glycine max
Sodium arsenate 1 mM, weak Glycine max
Tris buffer
-
Glycine max
UDP linear competitive inhibitor to UDPglucose Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.563
-
3,4-dichloroaniline
-
Glycine max
1.88
-
UDPglucose
-
Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Glycine max 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + an arylamine Glycine max involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism UDP + an N-D-glucosylarylamine
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone inactivates Glycine max
Ethanol inactivates Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
Merril, var. Hawkeye
-

Purification (Commentary)

Purification (Comment) Organism
partial Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Glycine max
-
hypocotyl
-
Glycine max
-
leaf
-
Glycine max
-
root
-
Glycine max
-
seed
-
Glycine max
-
seedling etiolated Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity in different tissues Glycine max
0.000912
-
-
Glycine max

Storage Stability

Storage Stability Organism
-15°C, lyophilized, several months Glycine max
4°C, t1/2: 48 h Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
TDP-glucose + an arylamine
-
Glycine max TDP + an N-D-glucosylarylamine
-
?
UDP-glucose + 2,3-dichloroaniline
-
Glycine max UDP + N-(2,3-dichlorophenyl)-glucosylamine
-
?
UDP-glucose + 2,4-dibromoaniline
-
Glycine max UDP + N-(2,4-dibromophenyl)-glucosylamine
-
?
UDP-glucose + 2,4-dichloroaniline
-
Glycine max UDP + N-(2,4-dichlorophenyl)-glucosylamine
-
?
UDP-glucose + 2,5-dibromoaniline
-
Glycine max UDP + N-(2,5-dibromophenyl)-glucosylamine
-
?
UDP-glucose + 2,5-dichloroaniline
-
Glycine max UDP + N-(2,5-dichlorophenyl)-glucosylamine
-
?
UDP-glucose + 3,4-dichloroaniline best glucosyl acceptor, no glucosyl donors are GDP-glucose, CDP-glucose, ADP-glucose, UDP-mannose Glycine max UDP + N-(3,4-dichlorophenyl)-glucosylamine
-
?
UDP-glucose + 3,5-dichloroaniline
-
Glycine max UDP + N-(3,5-dichlorophenyl)-glucosylamine
-
?
UDP-glucose + 3-amino-2,5-dichlorobenzoic acid
-
Glycine max UDP + 3-glucosylamino-2,5-dichlorobenzoic acid
-
?
UDP-glucose + 3-aminobenzoic acid
-
Glycine max UDP + 3-glucosylaminobenzoic acid
-
?
UDP-glucose + 3-chloroaniline 31% efficiency compared to 3,4-dichloroaniline Glycine max UDP + N-(3-chlorophenyl)-glucosylamine
-
?
UDP-glucose + 3-nitroaniline
-
Glycine max UDP + N-(3-nitrophenyl)-glucosylamine
-
?
UDP-glucose + 4-bromoaniline 28% efficiency compared to 3,4-dichloroaniline Glycine max UDP + N-(4-bromophenyl)-glucosylamine
-
?
UDP-glucose + 4-chloroaniline 71% efficiency compared to 3,4-dichloroaniline Glycine max UDP + N-(4-chlorophenyl)-glucosylamine
-
?
UDP-glucose + 5-amino-2,3-dichlorobenzoic acid
-
Glycine max UDP + 5-glucosylamino-2,3-dichlorobenzoic acid
-
?
UDP-glucose + an arylamine involved in N-glucosylarylamines biosynthesis, may be participate in arylamine herbicide metabolism Glycine max UDP + an N-D-glucosylarylamine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Glycine max
28
-
assay at Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
80% loss of activity within 10 min Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.8
-
Glycine max
7.5
-
with 3,4-dichloroaniline as acceptor Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.484
-
UDP
-
Glycine max