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Literature summary for 2.4.1.70 extracted from

  • Nathenson, S.G.; Ishimoto, N.; Strominger, J.L.
    UDP-N-acetylglucosamine: polyribitol phosphate N-acetylglucosaminyltransferase from Staphylococcus aureus (1966), Methods Enzymol., 8, 426-429.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ slight activation Staphylococcus aureus
Co2+ slight activation Staphylococcus aureus
Mg2+ 8 mM, required for activity Staphylococcus aureus
Mn2+ slight activation Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Staphylococcus aureus
Fe2+
-
Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
poly(ribitol phosphate)
-
Staphylococcus aureus
0.7
-
UDP-N-acetylglucosamine
-
Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + poly(ribitol phosphate) Staphylococcus aureus D-alanyl-N-acetyl-alpha-galactosamine poly(ribitol phosphate) is a less efficient acceptor, poor donor substrate is UDPglucose, no activity with teichoic acid, D-ribitol 5-phosphate or partial alkali hydrolysates of teichoic acid or N-acetyl-galactosamine polyribitol phosphate, UDP-N-acetyl-galactosamine, N-acetyl-galactosamine and N-acetyl-galactosamine phosphate UDP + N-acetyl-D-glucosaminyl-poly(ribitol phosphate)
-
ir

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
strains H or Duncan: primarily N-acetyl-beta-glucosamine transfer, strain 3528: N-acetyl-alpha-glucosamine transfer, strain Copenhagen: both transferases
-

Storage Stability

Storage Stability Organism
frozen, N-acetyl-alpha-D-glucosamine transferase, not N-acetyl-beta-D-glucosamine transferase, a few months Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + poly(ribitol phosphate) 10% of activity with UDP-N-acetyl-D-glucosamine Staphylococcus aureus UDP + glucosyl-poly(ribitol phosphate)
-
?
UDP-N-acetyl-D-glucosamine + poly(ribitol phosphate) D-alanyl-N-acetyl-alpha-galactosamine poly(ribitol phosphate) is a less efficient acceptor, poor donor substrate is UDPglucose, no activity with teichoic acid, D-ribitol 5-phosphate or partial alkali hydrolysates of teichoic acid or N-acetyl-galactosamine polyribitol phosphate, UDP-N-acetyl-galactosamine, N-acetyl-galactosamine and N-acetyl-galactosamine phosphate Staphylococcus aureus UDP + N-acetyl-D-glucosaminyl-poly(ribitol phosphate)
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Staphylococcus aureus