Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.7 extracted from

  • Yao, D.; Fan, J.; Han, R.; Xiao, J.; Li, Q.; Xu, G.; Dong, J.; Ni, Y.
    Enhancing soluble expression of sucrose phosphorylase in Escherichia coli by molecular chaperones (2020), Protein Expr. Purif., 169, 105571 .
    View publication on PubMed
Search for more literature for 2.4.1.7

Application

Application Comment Organism
industry the enzyme has a wide range of application in food, cosmetics, and pharmaceutical industries because of its broad substrate specificity Thermoanaerobacterium thermosaccharolyticum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3). To enhance the soluble expression of the enzyme, several chaperones plasmids such as pG-KJE8 (DnaK-DnaJ-GrpE-GroES-GroEL), pGro7 (GroES-GroEL), pKJE7 (DnaK-DnaJ-GrpE), and pG-TF2 (GroES-GroELTig) are coexpressed and their coexpression conditions are optimized Thermoanaerobacterium thermosaccharolyticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
55.6
-
 sucrose pH 6.5, 55°C Thermoanaerobacterium thermosaccharolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE Thermoanaerobacterium thermosaccharolyticum

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium thermosaccharolyticum WP_094046414.1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoanaerobacterium thermosaccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + hydroquinone
-
 Thermoanaerobacterium thermosaccharolyticum D-fructose + alpha-arbutin
-
 ?
sucrose + phosphate
-
 Thermoanaerobacterium thermosaccharolyticum D-fructose + alpha-D-glucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Thermoanaerobacterium thermosaccharolyticum

Synonyms

Synonyms Comment Organism
SPase
-
 Thermoanaerobacterium thermosaccharolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermoanaerobacterium thermosaccharolyticum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 45% of maximal activity, 70°C: about 85% of maximal activity Thermoanaerobacterium thermosaccharolyticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life: 19 min Thermoanaerobacterium thermosaccharolyticum
73
-
 melting temperature Thermoanaerobacterium thermosaccharolyticum
80
-
 half-life: 2.3 min Thermoanaerobacterium thermosaccharolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100
-
 sucrose pH 6.5, 55°C Thermoanaerobacterium thermosaccharolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Thermoanaerobacterium thermosaccharolyticum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity Thermoanaerobacterium thermosaccharolyticum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 1 h, the enzyme retains more than 50% of its activity Thermoanaerobacterium thermosaccharolyticum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
 sucrose pH 6.5, 55°C Thermoanaerobacterium thermosaccharolyticum