Application | Comment | Organism |
---|---|---|
synthesis | effective kojibiose synthesis i as performed using the crude enzyme solution from the supernatant of fermentation broth of Bacillus subtilis expressing the enzyme from Bifidobacterium adolescentis, providing a basis for potential industrial-scale preparation of kojibiose. Kojibiose is prebiotic element, a low-calorie sweetener and exhibits antitoxic activity, thereby promoting the emergence of new drugs | Bifidobacterium adolescentis |
Cloned (Comment) | Organism |
---|---|
heterologous expression in a food-grade Bacillus subtilis strain. Efficiently secreted into the extracellular medium in the absence of a signal peptide. After culturing the recombinant strain in a 3-l bioreactor, crude enzyme yield and activity reach 7.5 g/l and 5.3 U/ml, respectively | Bifidobacterium adolescentis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + D-glucose | kojibiose i.e. 2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranose | Bifidobacterium adolescentis | D-fructose + 2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranose | - |
? | |
sucrose + phosphate | - |
Bifidobacterium adolescentis | D-fructose + alpha-D-glucose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SPase | - |
Bifidobacterium adolescentis |