Literature summary for 2.4.1.7 extracted from

Bolivar, J.M.; Luley-Goedl, C.; Leitner, E.; Sawangwan, T.; Nidetzky, B.
Production of glucosyl glycerol by immobilized sucrose phosphorylase Options for enzyme fixation on a solid support and application in microscale flow format (2017), J. Biotechnol., 257, 131-138 .

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Application

Application	Comment	Organism
synthesis	2-O-(alpha-D-glucopyranosyl)-sn-glycerol (alphaGG) is a natural osmolyte. alphaGG is produced industrially for application as an active cosmetic ingredient. Immobilized preparations of the enzyme on Sepabeads and TRISOPERL controlled pore glass (CPG) supports are useful catalysts for alphaGG production, for they show the same high selectivity in the transglucosylation from sucrose to glycerol as the soluble enzyme does. Up to 90 mg enzyme loaded on solid support to give highly active immobilizate. Product yields of 85% and product titers of 800 mM reach at high reaction selectivity. High productivity (500 mM/h) obtained with immobilized enzyme in a microstructured flow reactor	Leuconostoc mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + glycerol	-	Leuconostoc mesenteroides	D-fructose + 2-O-(alpha-D-glucopyranosyl)-sn-glycerol	-	?
sucrose + phosphate	-	Leuconostoc mesenteroides	D-fructose + alpha-D-glucose 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SPase	-	Leuconostoc mesenteroides

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Release 2023.1 (January 2023)