Literature summary for 2.4.1.7 extracted from

Aerts, D.; Verhaeghe, T.; Joosten, H.J.; Vriend, G.; Soetaert, W.; Desmet, T.
Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input (2013), Biotechnol. Bioeng., 110, 2563-2572.

Search for more literature for 2.4.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Streptococcus mutans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics	Streptococcus mutans
18	-	sucrose	pH 6.5, 55°C	Streptococcus mutans
31	-	phosphate	pH 6.5, 55°C	Streptococcus mutans
33	-	alpha-D-glucose 1-phosphate	pH 6.5, 55°C	Streptococcus mutans
52	-	D-fructose	pH 6.5, 55°C	Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + phosphate	Streptococcus mutans	-	D-fructose + alpha-D-glucose 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptococcus mutans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Streptococcus mutans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose + alpha-D-glucose 1-phosphate	-	Streptococcus mutans	sucrose + phosphate	-	r
additional information	substrate specificity of the recombinant enzyme with alpha-Glc 1-phosphate as donor and acceptor, that is D-fructose, D-glucose, D-galactose, D-mannose, D-psicose, D-tagatose, or L-sorbose	Streptococcus mutans	?	-	?
sucrose + phosphate	-	Streptococcus mutans	D-fructose + alpha-D-glucose 1-phosphate	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Streptococcus mutans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
62	-	alpha-D-glucose 1-phosphate	pH 6.5, 55°C	Streptococcus mutans
66	-	D-fructose	pH 6.5, 55°C	Streptococcus mutans
127	-	sucrose	pH 6.5, 55°C	Streptococcus mutans
153	-	phosphate	pH 6.5, 55°C	Streptococcus mutans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Streptococcus mutans

General Information

General Information	Comment	Organism
evolution	sucrose phosphorylase (SP) is classified in subfamily 18 of the alpha-amylase family, GH13	Streptococcus mutans
additional information	homology structure modeling of the enzyme using the crystal structure of the closely related sucrose phosphorylase from Bifidobacterium adolescentis as model templates, PDB IDs 1R7A, 2GDV, and 2GDU	Streptococcus mutans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.3	-	D-fructose	pH 6.5, 55°C	Streptococcus mutans
1.9	-	alpha-D-glucose 1-phosphate	pH 6.5, 55°C	Streptococcus mutans
4.9	-	phosphate	pH 6.5, 55°C	Streptococcus mutans
7.1	-	sucrose	pH 6.5, 55°C	Streptococcus mutans

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Release 2023.1 (January 2023)