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Literature summary for 2.4.1.7 extracted from

  • Du, L.; Yang, H.; Huo, Y.; Wei, H.; Xu, Y.; Wei, Y.; Huang, R.
    A novel sucrose phosphorylase from the metagenomes of sucrose-rich environment: isolation and characterization (2012), World J. Microbiol. Biotechnol., 28, 2871-2878.
    View publication on PubMed
Search for more literature for 2.4.1.7

Cloned(Commentary)

Cloned (Comment) Organism
unspase, genetic library construction, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged unspase in Escherichia coli strain XL-1 Blue uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21.12
-
 sucrose pH 6.5, 37°C, recombinant enzyme uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + phosphate uncultured bacterium
-
 D-fructose + alpha-D-glucose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured bacterium B8Y3Y0 isolated from soil collected from a sugar refinery in Wuming, Guangxi Province, China
-

Purification (Commentary)

Purification (Comment) Organism
recombinant unspase from Escherichia coli strain XL-1 Blue by affinity chromatography uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the unspase is a sucrose phosphorylase able to catalyze the transglycosylation of different monomeric sugars, L-arabinose, D-fructose and L-sorbose, resulting in a 38% conversion rate uncultured bacterium ?
-
 ?
sucrose + L-arabinose
-
 uncultured bacterium D-fructose + ? the enzyme transglucosylated L-arabinose even in phosphate buffer ?
sucrose + L-sorbose
-
 uncultured bacterium D-fructose + ?
-
 ?
sucrose + phosphate
-
 uncultured bacterium D-fructose + alpha-D-glucose 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
unspase
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 purified enzyme, 10 min, inactivation uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.19
-
 sucrose pH 6.5, 37°C, recombinant enzyme uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 activity drops sharply below pH 5.5 uncultured bacterium

General Information

General Information Comment Organism
additional information compared with Bisp, the sucrose phosphorylase from Bifidobacterium adolescentis, unspase has two deleted regions in its C-terminal. These deleted regions are probably equivalent to the important five-stranded anti-parallel beta-sheet domain in sucrose phosphorylase uncultured bacterium
physiological function sucrose phosphorylase is an important enzyme mainly involved in the generic starch and sucrose pathways uncultured bacterium