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Literature summary for 2.4.1.69 extracted from

  • Pettit, N.; Styslinger, T.; Mei, Z.; Han, W.; Zhao, G.; Wang, P.G.
    Characterization of WbiQ: An alpha1->2-fucosyltransferase from Escherichia coli O127:K63(B8), and synthesis of H-type 3 blood group antigen (2010), Biochem. Biophys. Res. Commun., 402, 190-195.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.69

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme exhibits full activity when 10 mM EDTA is present Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
the GST-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
 Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require the presence of a divalent metal to be enzymatically active Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, GST-tagged enzyme, SDS-PAGE Escherichia coli
61000
-
 2 * 61000, calculated from amino acid sequence Escherichia coli
120000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q5J7C6
-
-
Escherichia coli O127:K63(B8) Q5J7C6
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OH
-
 Escherichia coli GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OH
-
 ?
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OH
-
 Escherichia coli O127:K63(B8) GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OH
-
 ?
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OMe highest activity Escherichia coli GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OMe
-
 ?
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OMe highest activity Escherichia coli O127:K63(B8) GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OMe
-
 ?
additional information the enzyme displays strict substrate specificity, displaying activity only towards acceptors that contain Gal-beta(1->3)-GalNAc-apha-OR linkages and does not recognize lactose, lactulose, Gal-alpha(1->3)-Gal-beta(1->4)Glc, alpha-GalGal-beta(1->4)-glucitol, and beta-D-Gal as acceptors Escherichia coli ?
-
 ?
additional information the enzyme displays strict substrate specificity, displaying activity only towards acceptors that contain Gal-beta(1->3)-GalNAc-apha-OR linkages and does not recognize lactose, lactulose, Gal-alpha(1->3)-Gal-beta(1->4)Glc, alpha-GalGal-beta(1->4)-glucitol, and beta-D-Gal as acceptors Escherichia coli O127:K63(B8) ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, GST-tagged enzyme, SDS-PAGE Escherichia coli
homodimer 2 * 61000, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
alpha1->2-fucosyltransferase
-
 Escherichia coli
alpha1->2-FucT
-
 Escherichia coli
WbiQ
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Escherichia coli