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Literature summary for 2.4.1.69 extracted from

  • Li, M.; Liu, X.W.; Shao, J.; Shen, J.; Jia, Q.; Yi, W.; Song, J.K.; Woodward, R.; Chow, C.S.; Wang, P.G.
    Characterization of a novel alpha1,2-fucosyltransferase of Escherichia coli O128:b12 and functional investigation of its common motif (2008), Biochemistry, 47, 378-387.
    View publication on PubMed
Search for more literature for 2.4.1.69

Cloned(Commentary)

Cloned (Comment) Organism
the wbsJ gene is overexpressed in Escherichia coli BL21 as a fusion protein with glutathione S-transferase (GST) at its N-terminus Escherichia coli

Protein Variants

Protein Variants Comment Organism
H152A Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose Escherichia coli
H152R Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose Escherichia coli
R154K Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose Escherichia coli
R155A Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose Escherichia coli
R155K Km increased compared to wild-type using substrate lactulose and substrate GDP-fucose Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Escherichia coli
GDP
-
 Escherichia coli
Ni2+
-
 Escherichia coli
Zn2+
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.106
-
 GDP-beta-L-fucose wild-type Escherichia coli
0.462
-
 GDP-beta-L-fucose mutant H152R Escherichia coli
0.575
-
 GDP-beta-L-fucose mutant R154K Escherichia coli
0.628
-
 GDP-beta-L-fucose mutant R155K Escherichia coli
0.865
-
 GDP-beta-L-fucose mutant H152A Escherichia coli
1.997
-
 GDP-beta-L-fucose mutant R155A Escherichia coli
7.81
-
 lactose wild-type Escherichia coli
13.26
-
 lactulose wild-type Escherichia coli
55.3
-
 lactulose mutant H152A Escherichia coli
59.62
-
 Galbeta-O-Me wild-type Escherichia coli
64.8
-
 lactulose mutant R155K Escherichia coli
76
-
 lactulose mutant R155A Escherichia coli
96.7
-
 lactulose mutant H152R Escherichia coli
108
-
 lactulose mutant R154K Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is found to be independent of divalent metal ions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST-WbsJ fusion protein is purified to homogeneity via GST affinity chromatography followed by gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 relative activity: lactose: 100%, Galbeta(1,4)Glcbeta-N3 (LacN3): 137%, Galbeta(1,4)Glcbeta-O-phenyl: 84.7%, Galbeta(1,4)Glcbeta-S-phenyl: 207%, Galbeta(1,4Glc)beta-1-NAc: 199%, Galbeta-p-nitrophenol: 30.4%, galactose: 35.7%, Galbeta-O-Me: 68.8%, Galbeta(1,4)GlcNAc: 12.4%, Galbeta(1,4)Fru: 380%, Galbeta(1,4)Man: 162%, Galbeta(1,3)GalNAcalpha-O-benzyl: 202%, Galbeta(1,3)GalNAcalpha-O-Me: 215% Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + galactose
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1,3)GalNAcalpha-O-Me
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1,3)GalNAcalpha-O-phenyl
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-1-NAc
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-N3
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-O-phenyl
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1,4)Glcbeta-S-phenyl
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1,4)Man
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + Galbeta(1-3)GalNAc
-
 Escherichia coli GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
-
 ?
GDP-beta-L-fucose + Galbeta-O-Me
-
 Escherichia coli GDP + L-Fucalpha(1-2)Galbeta-O-Me
-
 ?
GDP-beta-L-fucose + Galbeta-p-nitrophenol
-
 Escherichia coli GDP + L-Fucalpha(1-2)Galbeta-p-nitrophenol
-
 ?
GDP-beta-L-fucose + lactose
-
 Escherichia coli GDP + fucosyllactose
-
 ?
GDP-beta-L-fucose + lactulose
-
 Escherichia coli GDP + ?
-
 ?
GDP-beta-L-fucose + lactulose
-
 Escherichia coli GDP + 2'-fucosyl-D-lactulose
-
 ?

Subunits

Subunits Comment Organism
dimer gel filtration, 2 * 60000 Da Escherichia coli

Synonyms

Synonyms Comment Organism
alpha1,2-fucosyltransferase
-
 Escherichia coli
WbsJ
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0173
-
 methyl beta-D-galactopyranoside
-
 Escherichia coli
0.0497
-
 lactose
-
 Escherichia coli
0.131
-
 GDP-beta-L-fucose
-
 Escherichia coli
0.151
-
 lactulose
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.41
-
 GDP
-
 Escherichia coli