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Literature summary for 2.4.1.69 extracted from
- Enzymatic properties of the beta-galactoside alpha 1->2 fucosyltransferase from porcine submaxillary gland (1980), J. Biol. Chem., 255, 5373-5379.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of fut1, fut2, and sec1 genes in COS-7 cells | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 10 mM, complete inhibition | Sus scrofa |  |
| Cd2+ | 10 mM, complete inhibition | Sus scrofa | |
| Cu2+ | 10 mM, complete inhibition | Sus scrofa | |
| GMP | competitive with GDP-L-fucose | Sus scrofa | |
| Hg2+ | 10 mM, complete inhibition | Sus scrofa | |
| lactose | competitive with antifreeze glycoprotein | Sus scrofa | |
| Ni2+ | 10 mM | Sus scrofa | |
| Zn2+ | 10 mM, complete inhibition | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
Sus scrofa | |
| 0.28 | - |
Galbeta(1-3)GalNAc | - |
Sus scrofa | |
| 0.63 | - |
Galbeta(1-3)GlcNAc | - |
Sus scrofa | |
| 0.94 | - |
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta1-4Glc | - |
Sus scrofa | |
| 1.95 | - |
Galbeta(1-6)GalNAc | - |
Sus scrofa | |
| 2.11 | - |
Galbeta(1-4)GlcNAc | - |
Sus scrofa | |
| 2.56 | - |
Galbeta(1-6)GlcNAc | - |
Sus scrofa | |
| 10.7 | - |
Galbeta1-4Glc | - |
Sus scrofa | |
| 14.6 | - |
Galbeta(1-3)Ara | - |
Sus scrofa | |
| 18 | - |
GalbetaCH3 | - |
Sus scrofa | |
| 66 | - |
GalalphaCH3 | - |
Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa | |
| Co2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa | |
| Mg2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa | |
| Mn2+ | the enzyme is active in absence of divalent metal ions, but is stimulated by Mn2+, Mg2+, Ca2+ or Co2+ | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R | either a rapid equilibrium random kinetic mechanism or a steady state ordered mechanism with GDP-L-fucose binding first | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submaxillary gland | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-beta-L-fucose + Galbeta(1-3)GlcNAc | - |
Sus scrofa | ? | - |
? | |
| GDP-beta-L-fucose + Galbeta(1-6)GlcNAc | - |
Sus scrofa | ? | - |
? | |
| GDP-L-fucose + beta-D-galactosyl-R | the enzyme forms the Fuc(1-2)Gal linkage with oligosaccharides, glycoproteins, and glycolipids which contain nonreducing terminal galactose residues and shows no absolute specificity for a particular penultimate residue or for the linkage between the galactose and the penultimate residue. The enzyme fucosylates human Bombay" erythrocytes which lack cell surface Fucalpha1-2Gal structures | Sus scrofa | GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R | - |
? | |
| GDP-L-fucose + GalalphaCH3 | 0.1% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)GalalphaCH3 | - |
? | |
| GDP-L-fucose + Galbeta(1-3)Ara | 3.3% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)Ara | - |
? | |
| GDP-L-fucose + Galbeta(1-3)GalNAc | - |
Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc | - |
? | |
| GDP-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | 84% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
? | |
| GDP-L-fucose + Galbeta(1-4)Glc | 4.2% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc | - |
? | |
| GDP-L-fucose + Galbeta(1-4)GlcNAc | 7.5% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc | - |
? | |
| GDP-L-fucose + Galbeta(1-4)GlcNAc(1-3)Galbeta(1-4)Glc | 2.8% of the activity with Galbeta(1-3)GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAc(1-3)Glabeta(1-4)Glc | - |
? | |
| GDP-L-fucose + Galbeta(1-4)Man | 2.7% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-4)Man | - |
? | |
| GDP-L-fucose + Galbeta(1-6)GalNAc | 4.8% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc | - |
? | |
| GDP-L-fucose + GalbetaCH3 | 2.6% of the activity with Galbeta1-3GalNAc | Sus scrofa | GDP + L-Fucalpha(1-2)GalbetaCH3 | - |
? | |
| GDP-L-fucose + GM1 ganglioside | - |
Sus scrofa | GDP + ? | - |
? | |
| GDP-L-fucose + lacto-N-biose 1 | - |
Sus scrofa | GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc | - |
? | |
| GDP-L-fucose + lacto-N-neotetraose | - |
Sus scrofa | GDP + lacto-N-fucopentaose IV | - |
? | |
| GDP-L-fucose + paragloboside | - |
Sus scrofa | GDP + ? | - |
? | |
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