Application | Comment | Organism |
---|---|---|
synthesis | mutants of the general acid/base residue E274, including E274A, E274S, and E274G, act as efficient glycoligases able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using alpha-fucosyl fluoride as a simple donor substrate. The alpha-1,6-fucosidase mutants can introduce an alpha1,6-fucose moiety specifically at the Asn-linked GlcNAc moiety to GlcNAc-peptide, and also to high-mannose and complex type N-glycans in the context of N-glycopeptides, N-glycoproteins, and intact antibodies | Lacticaseibacillus casei |
Protein Variants | Comment | Organism |
---|---|---|
D200A | mutation at the nucleophilic residue, does not provide a typical glycosynthase activity | Lacticaseibacillus casei |
E274A | mutation at the general acid/base residue, mutant acts as efficient glycoligase able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins | Lacticaseibacillus casei |
E274G | mutation at the general acid/base residue, mutant acts as efficient glycoligase able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins | Lacticaseibacillus casei |
E274S | mutation at the general acid/base residue, mutant acts as efficient glycoligase able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins | Lacticaseibacillus casei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | - |
- |
- |
Lacticaseibacillus casei W56 | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
alfC | - |
Lacticaseibacillus casei |
BN194_28780 | - |
Lacticaseibacillus casei |