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Literature summary for 2.4.1.68 extracted from
- Purification and characterization of GDP-L-Fuc:N-acetyl beta-D-glucosaminide alpha1->6 fucosyltransferase from human blood platelets (1998), Glycoconjugate J., 15, 783-788.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme of blood platelets is a diagnostic marker for the ploidy level of megakaryocytes in thrombocytopenia | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | inactivation | Homo sapiens |  |
| Cu2+ | strong inhibition | Homo sapiens | |
| Ni2+ | strong inhibition | Homo sapiens | |
| Zn2+ | strong inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
GDP-L-fucose | - |
Homo sapiens | |
| 0.029 | - |
asialo-agalacto-transferrin-glycopeptide | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Homo sapiens | |
| Mg2+ | activation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from blood platelets by affinity chromatography with an asialo-agalacto-transferrin-glycopeptide resin | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood platelet | thrombin-activated neutrophils release cathepsin G and elastase during coagulation of blood, which promotes the degranulation of platelets and hence the secretion of alpha-6-fucosyltransferase | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
kinetics | Homo sapiens |
| 0.455 | - |
purified enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-L-fucose + asialo-agalactotransferrin glycopeptide | biantennary to tetraantennary substrates, with the triantennary substrate showing the highest activity | Homo sapiens | GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the asialo-agalactotransferrin glycopeptide | - |
? | |
| additional information | beta-D-mannosyl-(1-6)-[alpha-D-mannosyl-(1-3)-]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminoyl-asparagine is no substrate | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | - |
Homo sapiens |
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