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Literature summary for 2.4.1.68 extracted from

  • Wang, Y.; Spellman, M.W.
    Purification and characterization of a GDP-fucose:polypeptide fucosyltransferase from Chinese hamster ovary cells (1998), J. Biol. Chem., 273, 8112-8118.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Cricetulus griseus
Fe2+
-
Cricetulus griseus
Zn2+
-
Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
GDP-L-fucose
-
Cricetulus griseus
0.011
-
factor VII EGF-1 domain His-tagged substrate Cricetulus griseus
0.015
-
factor VII EGF-1 domain
-
Cricetulus griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Cricetulus griseus
Cd2+ activation Cricetulus griseus
Co2+ activation Cricetulus griseus
Mg2+ activation Cricetulus griseus
Mn2+ activation, 17fold at 50 mM Cricetulus griseus
additional information no effect of EDTA Cricetulus griseus
Ni2+ activation Cricetulus griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains more than 1 high-mannose type oligosaccharide chain with a total MW of 4000 Cricetulus griseus

Purification (Commentary)

Purification (Comment) Organism
-
Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.784
-
purified enzyme Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + factor VII EGF-1 domain recombinant human acceptor substrate, with and without His-tag Cricetulus griseus GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue ?
GDP-L-fucose + factor VII EGF-1 domain investigation of acceptor substrate specificity via site-directed mutagenesis of the factor VII EGF-1 domain leading to several structures, only some of which serve as substrates, overview Cricetulus griseus GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Cricetulus griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Cricetulus griseus