Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Cricetulus griseus | |
Fe2+ | - |
Cricetulus griseus | |
Zn2+ | - |
Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
GDP-L-fucose | - |
Cricetulus griseus | |
0.011 | - |
factor VII EGF-1 domain | His-tagged substrate | Cricetulus griseus | |
0.015 | - |
factor VII EGF-1 domain | - |
Cricetulus griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Cricetulus griseus | |
Cd2+ | activation | Cricetulus griseus | |
Co2+ | activation | Cricetulus griseus | |
Mg2+ | activation | Cricetulus griseus | |
Mn2+ | activation, 17fold at 50 mM | Cricetulus griseus | |
additional information | no effect of EDTA | Cricetulus griseus | |
Ni2+ | activation | Cricetulus griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains more than 1 high-mannose type oligosaccharide chain with a total MW of 4000 | Cricetulus griseus |
Purification (Comment) | Organism |
---|---|
- |
Cricetulus griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | - |
Cricetulus griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.784 | - |
purified enzyme | Cricetulus griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + factor VII EGF-1 domain | recombinant human acceptor substrate, with and without His-tag | Cricetulus griseus | GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain | fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue | ? | |
GDP-L-fucose + factor VII EGF-1 domain | investigation of acceptor substrate specificity via site-directed mutagenesis of the factor VII EGF-1 domain leading to several structures, only some of which serve as substrates, overview | Cricetulus griseus | GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain | fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Cricetulus griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cricetulus griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Cricetulus griseus |