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Literature summary for 2.4.1.68 extracted from
- Purification and characterization of a GDP-fucose:polypeptide fucosyltransferase from Chinese hamster ovary cells (1998), J. Biol. Chem., 273, 8112-8118.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Cricetulus griseus |  |
| Fe2+ | - |
Cricetulus griseus | |
| Zn2+ | - |
Cricetulus griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
GDP-L-fucose | - |
Cricetulus griseus | |
| 0.011 | - |
factor VII EGF-1 domain | His-tagged substrate | Cricetulus griseus | |
| 0.015 | - |
factor VII EGF-1 domain | - |
Cricetulus griseus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Cricetulus griseus | |
| Cd2+ | activation | Cricetulus griseus | |
| Co2+ | activation | Cricetulus griseus | |
| Mg2+ | activation | Cricetulus griseus | |
| Mn2+ | activation, 17fold at 50 mM | Cricetulus griseus | |
| additional information | no effect of EDTA | Cricetulus griseus | |
| Ni2+ | activation | Cricetulus griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 44000, SDS-PAGE | Cricetulus griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains more than 1 high-mannose type oligosaccharide chain with a total MW of 4000 | Cricetulus griseus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cricetulus griseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| CHO cell | - |
Cricetulus griseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.784 | - |
purified enzyme | Cricetulus griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-L-fucose + factor VII EGF-1 domain | recombinant human acceptor substrate, with and without His-tag | Cricetulus griseus | GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain | fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue | ? | |
| GDP-L-fucose + factor VII EGF-1 domain | investigation of acceptor substrate specificity via site-directed mutagenesis of the factor VII EGF-1 domain leading to several structures, only some of which serve as substrates, overview | Cricetulus griseus | GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain | fucose is attached through an o-glycosidic linkage to a conserved serine or threonine residue | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Cricetulus griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cricetulus griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Cricetulus griseus |
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