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Literature summary for 2.4.1.67 extracted from
- Stachyose synthesis in seeds of adzuki bean (Vigna angularis): molecular cloning and functional expression of stachyose synthase (1999), Plant J., 20, 509-518.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid determination, functional expression in Spodoptera frugiperda Sf21 insect cells via baculovirus infection | Vigna angularis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94900 | - |
DNA and amino acid sequence determination | Vigna angularis |
| 98000 | - |
1 * 98000, recombinant enzyme, SDS-PAGE | Vigna angularis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Spodoptera frugiperda | - |
- |
- |
| Vigna angularis | Q9SBZ0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna angularis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | mature | Vigna angularis | - |
| seed | expression and activity level during seed development | Vigna angularis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | - |
recombinant enzyme in Sf21 cells, substrates galactosylononitol and raffinose | Vigna angularis |
| 0.045 | - |
recombinant enzyme in Sf21 cells, substrates 1-alpha-D-galactosyl-myo-inositol and D-ononitol | Vigna angularis |
| 0.05 | - |
recombinant enzyme in Sf21 cells, substrates 1-alpha-D-galactosyl-myo-inositol and raffinose | Vigna angularis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-alpha-D-galactosyl-myo-inositol + D-ononitol | recombinant enzyme | Vigna angularis | myo-inositol + alpha-D-galactosyl-D-ononitol | - |
? |  |
| 1-alpha-D-galactosyl-myo-inositol + D-ononitol | ononitol is 1-D-4-O-methyl-myo-inositol | Vigna angularis | myo-inositol + alpha-D-galactosyl-D-ononitol | - |
? | |
| 1-alpha-D-galactosyl-myo-inositol + raffinose | native and recombinant enzyme | Vigna angularis | myo-inositol + stachyose | - |
? | |
| 1-alpha-D-galactosyl-myo-inositol + raffinose | i.e. galactinol | Vigna angularis | myo-inositol + stachyose | - |
? | |
| alpha-D-galactosyl-D-ononitol + raffinose | recombinant enzyme | Vigna angularis | D-ononitol + stachyose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 98000, recombinant enzyme, SDS-PAGE | Vigna angularis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| stachyose synthase | - |
Vigna angularis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Vigna angularis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.39 | Vigna angularis |
| 7 | - |
assay at | Vigna angularis |
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