Cloned (Comment) | Organism |
---|---|
full-length and each truncated FucT (DELTAFucT) are expressed in Escherichia coli HMS174DE3 | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
additional information | with full length UA948FucT, only 20% of total enzyme activities are localized in the soluble fraction. For UA948(1-428), this increases to 47%, respectively, indicating that truncation of the C-terminal putative alpha-helices increases FucT solubility. UA948(1-364), a mutant with the entire heptad repeat region removed, exhibits extremely low levels of enzyme activity. This indicates that the heptad repeat region is essential for enzyme activity. Construct UA948(1-371) has little enzyme activity. UA948(10-434) and UA948(1-434) completely lose activity | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0119 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, truncated enzyme UA948(1-428) | Helicobacter pylori | |
0.0337 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, full-length enzyme | Helicobacter pylori | |
5.7 | - |
Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | truncated enzyme UA948(1-428) | Helicobacter pylori | |
13.3 | - |
Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | full-length enzyme | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51200 | - |
51200, UA948FucT(1-428), calculated from sequence | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
UA948 | - |
Helicobacter pylori UA948 | - |
UA948 | - |
Purification (Comment) | Organism |
---|---|
UA948FucT(1-428) with a His6 tag at the C terminus is purified | Helicobacter pylori |
Storage Stability | Organism |
---|---|
-80°C, 3 months, lyophilized UA948FucT(1-428) loses 23% of its activity | Helicobacter pylori |
most stable at -80°C with or without glycerol supplementation | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-fucose + 3-sulfo-Galbeta1,3GlcNAc | 40% of the activity with Galbeta1,3GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + 3-sulfo-Galbeta1,3GlcNAc | 40% of the activity with Galbeta1,3GlcNAc | Helicobacter pylori NCTC11639 | ? | - |
? | |
GDP-beta-L-fucose + 3-sulfo-Galbeta1,3GlcNAc | 40% of the activity with Galbeta1,3GlcNAc | Helicobacter pylori UA948 | ? | - |
? | |
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,3GlcNAc | 34% of the activity with Galbeta1,3GlcNAc | Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,3GlcNAc | 34% of the activity with Galbeta1,3GlcNAc | Helicobacter pylori NCTC11639 | ? | - |
? | |
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,3GlcNAc | 34% of the activity with Galbeta1,3GlcNAc | Helicobacter pylori UA948 | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,3GlcNAc | - |
Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,3GlcNAc | - |
Helicobacter pylori NCTC11639 | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,3GlcNAc | - |
Helicobacter pylori UA948 | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | - |
Helicobacter pylori | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | - |
Helicobacter pylori NCTC11639 | ? | - |
? | |
GDP-beta-L-fucose + Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | - |
Helicobacter pylori UA948 | ? | - |
? | |
additional information | the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity | Helicobacter pylori | ? | - |
? | |
additional information | the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity | Helicobacter pylori NCTC11639 | ? | - |
? | |
additional information | the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity | Helicobacter pylori UA948 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 51200, UA948FucT(1-428), calculated from sequence | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
alpha-(1,3/1,4)-fucosyltransferase | - |
Helicobacter pylori |
alpha-(1,3/1,4)-FucT | - |
Helicobacter pylori |
UA948FucT | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
GDP-beta-L-fucose | cosubstrate: Galbeta1,3GlcNAc-O(CH2)8CO2CH3, truncated enzyme UA948(1-428) | Helicobacter pylori | |
1.1 | - |
Galbeta1,3GlcNAc-O(CH2)8CO2CH3 | truncated enzyme UA948(1-428) | Helicobacter pylori |