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Literature summary for 2.4.1.64 extracted from

  • Chen, C.; Van der Borght, J.; De Vreese, R.; Dhooghe, M.; Soetaert, W.; Desmet, T.
    Engineering the specificity of trehalose phosphorylase as a general strategy for the production of glycosyl phosphates (2014), Chem. Commun. (Camb.), 50, 7834-7836.
    View publication on PubMed
Search for more literature for 2.4.1.64

Protein Variants

Protein Variants Comment Organism
L649G the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme Caldanaerobacter subterraneus
L649G/A693Q the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme Caldanaerobacter subterraneus
L649G/A693Q/W371Y the mutant shows severely reduced production of beta-D-glucose 1-phosphate compared to the wild type enzyme but displays a strict specificity (99%) for beta-D-galactose 1-phosphate as product Caldanaerobacter subterraneus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 beta-D-galactose 1-phosphate mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
0.7
-
 beta-D-glucose 1-phosphate wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus
3.1
-
 beta-D-glucose 1-phosphate mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
3.7
-
 D-glucose wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus
3.8
-
 beta-D-galactose 1-phosphate wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus
4.6
-
 D-glucose mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
58
-
 D-galactose mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
125
-
 D-galactose wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + beta-D-glucose 1-phosphate Caldanaerobacter subterraneus
-
 lactotrehalose + phosphate
-
 r
lactotrehalose + phosphate Caldanaerobacter subterraneus
-
 D-galactose + beta-D-glucose 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate while the wild type enzyme shows very low activity, the mutant enzyme L649G/A693Q/W371Y displays a strict specificity (99%) for beta-D-galactose 1-phosphate as product Caldanaerobacter subterraneus D-glucose + beta-D-galactose 1-phosphate
-
 r
D-galactose + beta-D-glucose 1-phosphate
-
 Caldanaerobacter subterraneus lactotrehalose + phosphate
-
 r
D-glucose + beta-D-galactose 1-phosphate
-
 Caldanaerobacter subterraneus alpha-D-galactopyranosyl alpha-D-glucopyranoside + phosphate
-
 r
lactotrehalose + phosphate
-
 Caldanaerobacter subterraneus D-galactose + beta-D-glucose 1-phosphate
-
 r

Synonyms

Synonyms Comment Organism
trehalose phosphorylase
-
 Caldanaerobacter subterraneus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 D-galactose mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
1.2
-
 beta-D-galactose 1-phosphate wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus
1.6
-
 beta-D-galactose 1-phosphate mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
4.2
-
 D-glucose mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
7.4
-
 beta-D-glucose 1-phosphate mutant enzyme L649G/A693Q/W371Y, at pH 6.0 and 60°C Caldanaerobacter subterraneus
86
-
 D-glucose wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus
113
-
 D-galactose wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus
114
-
 beta-D-glucose 1-phosphate wild type enzyme, at pH 6.0 and 60°C Caldanaerobacter subterraneus