BRENDA - Enzyme Database
show all sequences of 2.4.1.64

Enzymatic properties and substrate specificity of the trehalose phosphorylase from Caldanaerobacter subterraneus

Van der Borght, J.; Chen, C.; Hoflack, L.; Van Renterghem, L.; Desmet, T.; Soetaert, W.; Appl. Environ. Microbiol. 77, 6939-6944 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli XL10 Gold cells
Caldanaerobacter subterraneus subsp. pacificus
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glucose
strong substrate inhibition, 500 mM D-glucose reduces the enzyme activity to 49.1% of the activity at the saturating concentration of 30 mM
Caldanaerobacter subterraneus subsp. pacificus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
20.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
64.8
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
171.5
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
183.4
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90487
-
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caldanaerobacter subterraneus subsp. pacificus
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA agarose resin column chromatography
Caldanaerobacter subterraneus subsp. pacificus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha,alpha-trehalose + phosphate
specific substrate
721346
Caldanaerobacter subterraneus subsp. pacificus
beta-D-glucose 1-phosphate + D-glucose
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-allose
4.5% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-arabinose
1.8% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-fucose
22% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-galactose
83.7% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-lyxose
1.3% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-mannose
14.1% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-xylose
135.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-L-fucose
109.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + beta-L-arabinose
79.9% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + D-glucose
100% activity with D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
alpha,alpha-trehalose + phosphate
-
-
-
r
additional information
the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
-
Caldanaerobacter subterraneus subsp. pacificus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
no loss of activity after 1 h of incubation at 65°C
Caldanaerobacter subterraneus subsp. pacificus
72
-
50% residual activity remains after 1 h of incubation at 72°C
Caldanaerobacter subterraneus subsp. pacificus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
51.4
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
54
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
59
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
64.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
68.1
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
the pH optimum is slightly different for both reaction directions, with a lower optimum (about pH 5.5) for the synthesis reaction
Caldanaerobacter subterraneus subsp. pacificus
7
-
the pH optimum is slightly different for both reaction directions, with a higher optimum (pH 7.0) for phosphorolysis
Caldanaerobacter subterraneus subsp. pacificus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
7
activity is over 90% in the range of pH 4.5 to 7.0
Caldanaerobacter subterraneus subsp. pacificus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Caldanaerobacter subterraneus subsp. pacificus
calculated from amino acid sequence
-
5.4
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli XL10 Gold cells
Caldanaerobacter subterraneus subsp. pacificus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glucose
strong substrate inhibition, 500 mM D-glucose reduces the enzyme activity to 49.1% of the activity at the saturating concentration of 30 mM
Caldanaerobacter subterraneus subsp. pacificus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
20.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
64.8
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
171.5
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
183.4
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
90487
-
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA agarose resin column chromatography
Caldanaerobacter subterraneus subsp. pacificus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha,alpha-trehalose + phosphate
specific substrate
721346
Caldanaerobacter subterraneus subsp. pacificus
beta-D-glucose 1-phosphate + D-glucose
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-allose
4.5% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-arabinose
1.8% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-fucose
22% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-galactose
83.7% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-lyxose
1.3% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-mannose
14.1% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-D-xylose
135.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + alpha-L-fucose
109.2% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + beta-L-arabinose
79.9% activity compared to D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
r
beta-D-glucose 1-phosphate + D-glucose
100% activity with D-glucose
721346
Caldanaerobacter subterraneus subsp. pacificus
alpha,alpha-trehalose + phosphate
-
-
-
r
additional information
the enzyme shows no measurable activity with alpha-D-glucosamine, alpha-D-ribose, D-maltose or D-kojibiose
721346
Caldanaerobacter subterraneus subsp. pacificus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 90487, calculated from amino acid sequence
Caldanaerobacter subterraneus subsp. pacificus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
-
Caldanaerobacter subterraneus subsp. pacificus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
-
no loss of activity after 1 h of incubation at 65°C
Caldanaerobacter subterraneus subsp. pacificus
72
-
50% residual activity remains after 1 h of incubation at 72°C
Caldanaerobacter subterraneus subsp. pacificus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
51.4
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
54
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
59
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
64.3
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
68.1
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
the pH optimum is slightly different for both reaction directions, with a lower optimum (about pH 5.5) for the synthesis reaction
Caldanaerobacter subterraneus subsp. pacificus
7
-
the pH optimum is slightly different for both reaction directions, with a higher optimum (pH 7.0) for phosphorolysis
Caldanaerobacter subterraneus subsp. pacificus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
7
activity is over 90% in the range of pH 4.5 to 7.0
Caldanaerobacter subterraneus subsp. pacificus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Caldanaerobacter subterraneus subsp. pacificus
calculated from amino acid sequence
-
5.4
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.315
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
0.371
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
0.793
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
3.17
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
17
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.315
-
alpha-D-galactose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
0.371
-
alpha-L-fucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
0.793
-
alpha-D-arabinose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
3.17
-
alpha-D-xylose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
17
-
D-glucose
in 50 mM MES buffer (pH 6.0), at 60°C
Caldanaerobacter subterraneus subsp. pacificus
Other publictions for EC 2.4.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735936
Chen
Engineering the specificity of ...
Caldanaerobacter subterraneus
Chem. Commun. (Camb.)
50
7834-7836
2014
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3
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8
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2
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1
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4
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8
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3
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8
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2
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4
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8
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736104
Nihira
Potassium ion-dependent trehal ...
Bacillus selenitireducens, Bacillus selenitireducens MLS10
FEBS Lett.
587
3382-3386
2013
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-
1
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1
4
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1
3
4
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4
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1
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4
1
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3
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1
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1
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1
1
4
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1
3
4
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1
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4
1
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3
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1
1
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721914
Van der Borght
Engineering the acceptor speci ...
Thermoanaerobacter brockii
Biotechnol. Prog.
28
1257-1262
2012
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-
1
-
20
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35
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2
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1
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3
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1
41
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1
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20
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35
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3
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1
41
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37
37
721346
Van der Borght
Enzymatic properties and subst ...
Caldanaerobacter subterraneus subsp. pacificus
Appl. Environ. Microbiol.
77
6939-6944
2011
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1
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1
5
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1
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4
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1
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12
1
1
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2
5
2
1
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1
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1
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1
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5
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1
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1
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12
1
1
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2
5
2
1
-
1
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5
5
721191
Van Hoorebeke
Crystallization and X-ray diff ...
Thermoanaerobacter brockii subsp. brockii, Thermoanaerobacter brockii subsp. brockii ATCC 33075
Acta Crystallogr. Sect. F
66
442-447
2010
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1
1
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3
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5
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1
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1
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1
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3
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1
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721896
Van der Borght
Enzymatic production of beta-D ...
Thermoanaerobacter brockii
Biotechnol. J.
5
986-993
2010
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1
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4
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1
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1
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1
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682140
Lopez
Increased trehalose biosynthes ...
Amanita muscaria
New Phytol.
174
389-398
2007
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4
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1
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693804
Lopienska-Biernat
Trehalose catabolism enzymes i ...
Anisakis simplex
J. Parasitol.
93
1291-1294
2007
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3
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1
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1
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2
1
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1
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1
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672930
Yamamoto
Construction and characterizat ...
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Trehalose catabolism enzymes i ...
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Gene encoding a trehalose phos ...
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489325
Aisaka
Production of trehalose phosph ...
Actinomadura fulvescens, Actinomadura luteofluorescens, Actinomadura verrucosospora, Asanoa ferruginea, Kineosporia aurantiaca, Nocardia coeliaca, Pseudonocardia autotrophica
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Enzymatic synthesis of trehalo ...
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Fructose 2,6-bisphosphate, a p ...
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Metabolism of trehalose in Eug ...
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489320
Belocopitow
Trehalose phosphorylase from E ...
Euglena gracilis
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