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Literature summary for 2.4.1.64 extracted from
- Purification and characterization of trehalose phosphorylase from Micrococcus varians (1995), Biosci. Biotechnol. Biochem., 59, 1908-1912.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxynojirimycin | 1 mM, complete inhibition | Kocuria varians |  |
| Cu2+ | 1 mM, complete inhibition | Kocuria varians | |
| Ni2+ | 1 mM, 88% inhibition | Kocuria varians | |
| validamycin A | 10 mM, complete inhibition | Kocuria varians | |
| validoxylamine A | 0.2 mM, complete inhibition | Kocuria varians | |
| Zn2+ | 1 mM, complete inhibition | Kocuria varians | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
phosphate | - |
Kocuria varians | |
| 10 | - |
trehalose | - |
Kocuria varians | |
| 23 | - |
glucose | - |
Kocuria varians | |
| 38 | - |
beta-D-glucose 1-phosphate | - |
Kocuria varians | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
6 * 105000, SDS-PAGE | Kocuria varians |
| 570000 | 580000 | gel filtration | Kocuria varians |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kocuria varians | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.2 | - |
- |
Kocuria varians |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + phosphate | - |
Kocuria varians | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 105000, SDS-PAGE | Kocuria varians |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
both directions | Kocuria varians |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below | Kocuria varians |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 200 | - |
phosphate | - |
Kocuria varians | |
| 200 | - |
trehalose | - |
Kocuria varians | |
| 660 | - |
glucose | - |
Kocuria varians | |
| 660 | - |
beta-D-glucose 1-phosphate | - |
Kocuria varians | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
synthesis of trehalose | Kocuria varians |
| 7 | - |
phosphorolysis of trehalose | Kocuria varians |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | stable | Kocuria varians |
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Release 2023.1 (January 2023)
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