General Stability | Organism |
---|---|
enzyme retains full activity during 5 months at -14°C with several freezings and thawings, in presence of 2 mM phosphate buffer, pH 7.0, beta-D-glucose-1-phosphate and alpha-D-glucose-1-phosphate | Euglena gracilis |
loss of activity when extracts are desalted by dialysis or passage through Sephadex G-25 in presence of water. When dialysis is carried out against 5 mM NaCl, glucose 6-phosphate, EDTA, or imidazole-HCl buffer, pH 7.0, the enzymatic activity is maintained only for a few h | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
beta-D-glucose 1-phosphate | - |
Euglena gracilis | |
9.4 | - |
phosphate | - |
Euglena gracilis | |
32 | - |
glucose | - |
Euglena gracilis | |
33 | - |
trehalose | - |
Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
344000 | - |
sucrose density gradient centrifugation | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + phosphate | Euglena gracilis | enzyme is involved in trehalose catabolism | D-glucose + beta-D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
var. bacillaris | - |
Purification (Comment) | Organism |
---|---|
partial | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.214 | - |
- |
Euglena gracilis |
Storage Stability | Organism |
---|---|
14°C,in presence of 2 mM phosphate buffer, pH 7.0, beta-D-glucose-1-phosphate and alpha-D-glucose-1-phosphate, enzyme retains full activity during 5 months with several freezings and thawings | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-deoxy-D-glucose + alpha-D-glucose 1-phosphate | at 93% the activity with rate of glucose glucosylation | Euglena gracilis | alpha-D-glucopyranosyl 6-deoxy-alpha-D-glucopyranoside + phosphate | - |
? | |
alpha,alpha-trehalose + phosphate | - |
Euglena gracilis | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
alpha,alpha-trehalose + phosphate | enzyme is involved in trehalose catabolism | Euglena gracilis | D-glucose + beta-D-glucose 1-phosphate | - |
? | |
xylose + beta-D-glucose 1-phosphate | glucosylated at 23% of the rate of glucose glucosylation | Euglena gracilis | glucosyl-1,1-xylose 1-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
synthesis of trehalose | Euglena gracilis |
7 | - |
- |
Euglena gracilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 7.8 | about half-maximal activity at pH 5.7 and 7.8, trehalose synthesis | Euglena gracilis |
6 | 8 | about half-maximal activity at pH 6 and 8, phosphorolysis | Euglena gracilis |