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Literature summary for 2.4.1.58 extracted from

  • Creeger, E.S.; Rothfield, L.I.
    Cloning of genes for bacterial glycosyltransferases. I. Selection of hybrid plasmids carrying genes for two glucosyltransferases (1979), J. Biol. Chem., 254, 804-810.
    View publication on PubMed
Search for more literature for 2.4.1.58

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Phospholipid activates, minimal activity in absence Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
rfaG gene encodes enzyme, use of hybrid ColE1 plasmids containing Escherichia coli genes for glycosyltransferases to introduce enzyme into Salmonella typhimurium strains SL1032/pL10-7 and pLC17-24 lacking enzyme, plasmids are capable of correcting the transferase defect Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 UDPglucose
-
 Escherichia coli
0.33
-
 UDPglucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
cytoplasmic membrane
-
 Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information requires divalent cations Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium involved in biosynthesis of cell wall lipopolysaccharide UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide Escherichia coli involved in biosynthesis of cell wall lipopolysaccharide UDP + D-glucosyllipopolysaccharide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 recombinant enzyme expressed in Salmonella typhimurium
-
Escherichia coli K12 CS520
-
 recombinant enzyme expressed in Salmonella typhimurium
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information no sugar donors: glucose, glucose-6-phosphate, galactose, CDPglucose, TDPglucose, ADPglucose, UDPgalactose, no sugar acceptor: lipopolysaccharide from wild-type Salmonella typhimurium SA722 Escherichia coli ?
-
 ?
additional information no sugar donors: glucose, glucose-6-phosphate, galactose, CDPglucose, TDPglucose, ADPglucose, UDPgalactose, no sugar acceptor: lipopolysaccharide from wild-type Salmonella typhimurium SA722 Escherichia coli K12 CS520 ?
-
 ?
UDPglucose + lipopolysaccharide core region of the lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide core region of the lipopolysaccharide Escherichia coli UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide transfers glucosyl residues to a heptose residue of lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide transfers glucosyl residues to a heptose residue of lipopolysaccharide Escherichia coli UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032 as glucose acceptor, incorporation of 0.27 mol glucose per mol of heptose in the acceptor lipopolysaccharide, 53% of the theoretical maximum incorporation Escherichia coli UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide specific for UDP-glucose Escherichia coli UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide involved in biosynthesis of cell wall lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide involved in biosynthesis of cell wall lipopolysaccharide Escherichia coli UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide core region of the lipopolysaccharide Escherichia coli K12 CS520 UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide transfers glucosyl residues to a heptose residue of lipopolysaccharide Escherichia coli K12 CS520 UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032 as glucose acceptor, incorporation of 0.27 mol glucose per mol of heptose in the acceptor lipopolysaccharide, 53% of the theoretical maximum incorporation Escherichia coli K12 CS520 UDP + D-glucosyllipopolysaccharide
-
 ?
UDPglucose + lipopolysaccharide specific for UDP-glucose Escherichia coli K12 CS520 UDP + D-glucosyllipopolysaccharide
-
 ?

Synonyms

Synonyms Comment Organism
UDPglucose:(heptosyl)lipopolysaccharide 1,3-glucosyltransferase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium
37
-
 assay at Escherichia coli