BRENDA - Enzyme Database show
show all sequences of 2.4.1.58

Cloning of genes for bacterial glycosyltransferases. I. Selection of hybrid plasmids carrying genes for two glucosyltransferases

Creeger, E.S.; Rothfield, L.I.; J. Biol. Chem. 254, 804-810 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Phospholipid
activates, minimal activity in absence
Escherichia coli
Phospholipid
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary)
Commentary
Organism
rfaG gene encodes enzyme, use of hybrid ColE1 plasmids containing Escherichia coli genes for glycosyltransferases to introduce enzyme into Salmonella typhimurium strains SL1032/pL10-7 and pLC17-24 lacking enzyme, plasmids are capable of correcting the transferase defect
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
UDPglucose
-
Escherichia coli
0.33
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
-
Escherichia coli
-
-
cytoplasmic membrane
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
requires divalent cations
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
UDPglucose + lipopolysaccharide
Escherichia coli
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
recombinant enzyme expressed in Salmonella typhimurium
-
Escherichia coli K12 CS520
-
recombinant enzyme expressed in Salmonella typhimurium
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
-
489300
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
no sugar donors: glucose, glucose-6-phosphate, galactose, CDPglucose, TDPglucose, ADPglucose, UDPgalactose, no sugar acceptor: lipopolysaccharide from wild-type Salmonella typhimurium SA722
489300
Escherichia coli
?
-
-
-
-
additional information
no sugar donors: glucose, glucose-6-phosphate, galactose, CDPglucose, TDPglucose, ADPglucose, UDPgalactose, no sugar acceptor: lipopolysaccharide from wild-type Salmonella typhimurium SA722
489300
Escherichia coli K12 CS520
?
-
-
-
-
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489300
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489300
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to a heptose residue of lipopolysaccharide
489300
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489300
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to a heptose residue of lipopolysaccharide
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032 as glucose acceptor, incorporation of 0.27 mol glucose per mol of heptose in the acceptor lipopolysaccharide, 53% of the theoretical maximum incorporation
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
specific for UDP-glucose
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489300
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to a heptose residue of lipopolysaccharide
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
UDPglucose + lipopolysaccharide
glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032 as glucose acceptor, incorporation of 0.27 mol glucose per mol of heptose in the acceptor lipopolysaccharide, 53% of the theoretical maximum incorporation
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
UDPglucose + lipopolysaccharide
specific for UDP-glucose
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Phospholipid
activates, minimal activity in absence
Escherichia coli
Phospholipid
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
Commentary
Organism
rfaG gene encodes enzyme, use of hybrid ColE1 plasmids containing Escherichia coli genes for glycosyltransferases to introduce enzyme into Salmonella typhimurium strains SL1032/pL10-7 and pLC17-24 lacking enzyme, plasmids are capable of correcting the transferase defect
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
UDPglucose
-
Escherichia coli
0.33
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
-
Escherichia coli
-
-
cytoplasmic membrane
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
requires divalent cations
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
UDPglucose + lipopolysaccharide
Escherichia coli
involved in biosynthesis of cell wall lipopolysaccharide
UDP + D-glucosyllipopolysaccharide
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
-
489300
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
no sugar donors: glucose, glucose-6-phosphate, galactose, CDPglucose, TDPglucose, ADPglucose, UDPgalactose, no sugar acceptor: lipopolysaccharide from wild-type Salmonella typhimurium SA722
489300
Escherichia coli
?
-
-
-
-
additional information
no sugar donors: glucose, glucose-6-phosphate, galactose, CDPglucose, TDPglucose, ADPglucose, UDPgalactose, no sugar acceptor: lipopolysaccharide from wild-type Salmonella typhimurium SA722
489300
Escherichia coli K12 CS520
?
-
-
-
-
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489300
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489300
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to a heptose residue of lipopolysaccharide
489300
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489300
Salmonella enterica subsp. enterica serovar Typhimurium
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to a heptose residue of lipopolysaccharide
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032 as glucose acceptor, incorporation of 0.27 mol glucose per mol of heptose in the acceptor lipopolysaccharide, 53% of the theoretical maximum incorporation
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
specific for UDP-glucose
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489300
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
involved in biosynthesis of cell wall lipopolysaccharide
489300
Escherichia coli
UDP + D-glucosyllipopolysaccharide
-
-
-
?
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
UDPglucose + lipopolysaccharide
transfers glucosyl residues to a heptose residue of lipopolysaccharide
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
UDPglucose + lipopolysaccharide
glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032 as glucose acceptor, incorporation of 0.27 mol glucose per mol of heptose in the acceptor lipopolysaccharide, 53% of the theoretical maximum incorporation
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
UDPglucose + lipopolysaccharide
specific for UDP-glucose
489300
Escherichia coli K12 CS520
UDP + D-glucosyllipopolysaccharide
-
489300
Escherichia coli K12 CS520
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 2.4.1.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719223
Twelkmeyer
The role of lic2B in lipopolys ...
Haemophilus influenzae, Haemophilus influenzae Eagan
Carbohydr. Res.
346
1262-1266
2011
-
-
1
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3
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1
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-
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-
720277
Perumal
Cloning and targeted disruptio ...
Pseudomonas aeruginosa
J. Mol. Microbiol. Biotechnol.
19
169-179
2010
-
-
1
-
-
-
-
-
-
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1
-
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1
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1
1
-
-
-
703886
Schwingel
A unique glycosyltransferase i ...
Moraxella catarrhalis
Glycobiology
18
447-455
2008
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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3
-
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3
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
489300
Creeger
Cloning of genes for bacterial ...
Escherichia coli, Escherichia coli K12 CS520, Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
254
804-810
1979
2
-
1
-
-
-
-
2
2
1
-
2
-
8
-
-
1
-
-
-
2
-
15
-
2
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
2
2
1
-
2
-
-
-
1
-
-
2
-
15
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
489212
Muller
Studies of phospholipid-requir ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium G30A
J. Biol. Chem.
247
2614-2622
1972
2
-
-
-
-
-
2
3
1
4
1
1
-
2
-
1
1
-
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
3
1
4
1
1
-
-
1
1
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489298
Rothfield
Biosynthesis of bacterial lipo ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SL797
J. Biol. Chem.
239
2788-2795
1964
-
-
-
-
-
1
-
2
1
2
-
2
-
2
-
-
-
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
2
-
2
-
-
-
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-