BRENDA - Enzyme Database
show all sequences of 2.4.1.54

Mannosylphosphoryldolichol-mediated reactions in oligosaccharide-P-P-dolichol biosynthesis. Recognition of the saturated alpha-isoprene unit of the mannosyl donor by pig brain mannosyltransferases

Rush, J.S.; Shelling, J.G.; Zingg, N.S.; Ray, P.H.; Waechter, C.J.; J. Biol. Chem. 268, 13110-13117 (1993)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Micrococcus luteus
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDPmannose + undecaprenyl phosphate
Micrococcus luteus
-
GDP + D-mannosyl-1-phosphoundecaprenol
Man-P-Undec is an activated mannosyl donor in micrococcal mannan biosynthesis
Micrococcus luteus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Micrococcus luteus
-
formerly Micrococcus lysodeikticus
-
Purification (Commentary)
Commentary
Organism
partial
Micrococcus luteus
Storage Stability
Storage Stability
Organism
-20C, at least 1 year, stable
Micrococcus luteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + citronellyl phosphate
-
489291
Micrococcus luteus
GDP + mannosyl-phosphorylcitronellol
beta-isomer as product
489291
Micrococcus luteus
?
GDPmannose + dolichyl phosphate
-
489291
Micrococcus luteus
GDP + mannosyl-phosphoryldolichol
beta-isomer as product
489291
Micrococcus luteus
?
GDPmannose + polyisoprenyl phosphate
mannosylates a wide range of isoprenyl monophosphate substrates
489291
Micrococcus luteus
GDP + mannosyl-phosphorylpolyisoprenol
beta-isomer as product
489291
Micrococcus luteus
?
GDPmannose + undecaprenyl phosphate
-
489291
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
-
489291
Micrococcus luteus
?
GDPmannose + undecaprenyl phosphate
-
489291
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
Man-P-Undec is an activated mannosyl donor in micrococcal mannan biosynthesis
489291
Micrococcus luteus
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay at room temperature
Micrococcus luteus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Micrococcus luteus
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDPmannose + undecaprenyl phosphate
Micrococcus luteus
-
GDP + D-mannosyl-1-phosphoundecaprenol
Man-P-Undec is an activated mannosyl donor in micrococcal mannan biosynthesis
Micrococcus luteus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Micrococcus luteus
Storage Stability (protein specific)
Storage Stability
Organism
-20C, at least 1 year, stable
Micrococcus luteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDPmannose + citronellyl phosphate
-
489291
Micrococcus luteus
GDP + mannosyl-phosphorylcitronellol
beta-isomer as product
489291
Micrococcus luteus
?
GDPmannose + dolichyl phosphate
-
489291
Micrococcus luteus
GDP + mannosyl-phosphoryldolichol
beta-isomer as product
489291
Micrococcus luteus
?
GDPmannose + polyisoprenyl phosphate
mannosylates a wide range of isoprenyl monophosphate substrates
489291
Micrococcus luteus
GDP + mannosyl-phosphorylpolyisoprenol
beta-isomer as product
489291
Micrococcus luteus
?
GDPmannose + undecaprenyl phosphate
-
489291
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
-
489291
Micrococcus luteus
?
GDPmannose + undecaprenyl phosphate
-
489291
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
Man-P-Undec is an activated mannosyl donor in micrococcal mannan biosynthesis
489291
Micrococcus luteus
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay at room temperature
Micrococcus luteus
Other publictions for EC 2.4.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
681770
Rush
Partial purification of mannos ...
Micrococcus luteus
Methods Mol. Biol.
347
13-30
2006
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489291
Rush
Mannosylphosphoryldolichol-med ...
Micrococcus luteus
J. Biol. Chem.
268
13110-13117
1993
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
1
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
489289
Clark
An artificial mannosyl accepto ...
Vigna radiata var. radiata
FEBS Lett.
32
84-86
1973
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
489290
Forsee
Biosynthesis of mannosyl- and ...
Gossypium hirsutum, Mycolicibacterium smegmatis
J. Biol. Chem.
248
2858-2867
1973
-
-
-
-
-
-
1
2
2
2
-
1
-
2
-
-
-
-
-
1
-
-
6
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
2
-
1
-
-
-
-
-
1
-
-
6
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
489288
Lahav
Studies on the biosynthesis of ...
Micrococcus luteus
J. Biol. Chem.
244
5890-5898
1969
7
-
-
-
-
-
3
-
-
4
-
1
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
1
-
-
-
-
-
7
-
-
-
-
-
-
-
3
-
-
-
4
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-