Any feedback?
Literature summary for 2.4.1.54 extracted from
- Biosynthesis of mannosyl- and glucosyl-phosphoryl-polyprenols in cotton fibers (1973), J. Biol. Chem., 248, 2858-2867.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | at high concentrations | Gossypium hirsutum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00067 | - |
GDPmannose | - |
Gossypium hirsutum | |
| 0.02 | 0.04 | ficaprenyl phosphate | - |
Gossypium hirsutum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Mycolicibacterium smegmatis | 16020 | - |
| membrane | membrane-bound | Gossypium hirsutum | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Mycolicibacterium smegmatis | |
| Mg2+ | requirement | Gossypium hirsutum | |
| Mg2+ | optimal concentration: 15 mM, facilitates interaction of lipid and enzyme | Mycolicibacterium smegmatis | |
| Mg2+ | optimal concentration: 15 mM, facilitates interaction of lipid and enzyme | Gossypium hirsutum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDPmannose + phospholipid | Gossypium hirsutum | polyprenol type, involved in glycophospholipid biosynthesis | GDP + mannosyl-1-phospholipid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gossypium hirsutum | - |
12-15 days after anthesis | - |
| Mycolicibacterium smegmatis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fiber | - |
Gossypium hirsutum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDPmannose + ficaprenyl phosphate | effective substrate | Gossypium hirsutum | GDP + mannosyl-1-phosphorylficaprenol | - |
? | |
| GDPmannose + phospholipid | polyprenol type, involved in glycophospholipid biosynthesis | Gossypium hirsutum | GDP + mannosyl-1-phospholipid | - |
? | |
| GDPmannose + polyisoprenyl phosphate | endogenous lipid moiety of the glycolipid is a polyprenol and similar to undecaprenyl phosphate, but probably not identical with it, reaction is reversed by GDP, but not by GMP | Gossypium hirsutum | GDP + mannosyl-phosphorylpolyisoprenol | - |
r | |
| GDPmannose + undecaprenyl phosphate | reaction is reversed by GDP | Mycolicibacterium smegmatis | GDP + D-mannosyl-1-phosphoundecaprenol | - |
r | |
| additional information | no substrates: phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, Triton X-100 | Gossypium hirsutum | ? | - |
? | |
| UDPglucose + ficaprenyl phosphate | in the reverse reaction UMP is no substrate | Gossypium hirsutum | GDP + glucosyl-1-phosphorylficaprenol | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Gossypium hirsutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
Tris-maleate buffer | Gossypium hirsutum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 9 | Tris buffer, about half-maximal activity at pH 6.2 and 63% of maximal activity at pH 9.0 | Gossypium hirsutum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
