KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
poly(ribitol phosphate) | molar concentration of the polymer | Bacillus subtilis | |
0.017 | 0.08 | poly(ribitol phosphate) | concentration as ribitol units | Bacillus subtilis | |
0.12 | - |
UDP-D-glucose | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | bound, enzyme content is constant at all stages of growth | Bacillus subtilis | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | absolute requirement for the addition of a divalent cation, optimal concentration: 0.01 M | Bacillus subtilis | |
MgCl2 | absolute requirement for the addition of a divalent cation, optimal concentration: 0.05 M | Bacillus subtilis | |
MnCl2 | absolute requirement for the addition of a divalent cation, optimal concentration 0.01 M | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose + poly(ribitol phosphate) | Bacillus subtilis | biosynthesis of teichoic acids | UDP + glucosyl-poly(ribitol phosphate) | - |
? | |
UDP-D-glucose + poly(ribitol phosphate) | Bacillus subtilis W-23 | biosynthesis of teichoic acids | UDP + glucosyl-poly(ribitol phosphate) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
W-23 | - |
Bacillus subtilis W-23 | - |
W-23 | - |
Purification (Comment) | Organism |
---|---|
W-23, partial | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, crude preparation, stable for several months | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose + poly(ribitol phosphate) | polyglycerol phosphate is no substrate | Bacillus subtilis | UDP + glucosyl-poly(ribitol phosphate) | - |
? | |
UDP-D-glucose + poly(ribitol phosphate) | biosynthesis of teichoic acids | Bacillus subtilis | UDP + glucosyl-poly(ribitol phosphate) | - |
? | |
UDP-D-glucose + poly(ribitol phosphate) | polyglycerol phosphate is no substrate | Bacillus subtilis W-23 | UDP + glucosyl-poly(ribitol phosphate) | - |
? | |
UDP-D-glucose + poly(ribitol phosphate) | biosynthesis of teichoic acids | Bacillus subtilis W-23 | UDP + glucosyl-poly(ribitol phosphate) | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 62% of maximal activity at pH 6.0, 70% of maximal activity at pH 8.0 | Bacillus subtilis |