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Literature summary for 2.4.1.5 extracted from

  • Diez-Municio, M.; Herrero, M.; Jimeno, M.L.; Olano, A.; Moreno, F.J.
    Efficient synthesis and characterization of lactulosucrose by Leuconostoc mesenteroides B?512F dextransucrase (2012), J. Agric. Food Chem., 60, 10564-10571.
    View publication on PubMed
Search for more literature for 2.4.1.5

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides B?512F
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
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-
-
Leuconostoc mesenteroides B?512F
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation purified enzyme Leuconostoc mesenteroides
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
 commercial preparation, pH 5.2, 30°C Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also catalyzes the hydrolysis of sucrose to give D-glucose and D-fructose. Leucrose (alpha-D-Glu-(1->5)-D-Fru) formation is attributed to the minor capacity of free D-fructose to act as acceptor in the dextransucrase-catalyzed reactions, formation of leucrose by action of dextransucrase is favored at high fructose concentration. Once the availability of sucrose is very limited, lactulosucrose can then act as donor substrate Leuconostoc mesenteroides ?
-
 ?
additional information the enzyme also catalyzes the hydrolysis of sucrose to give D-glucose and D-fructose. Leucrose (alpha-D-Glu-(1->5)-D-Fru) formation is attributed to the minor capacity of free D-fructose to act as acceptor in the dextransucrase-catalyzed reactions, formation of leucrose by action of dextransucrase is favored at high fructose concentration. Once the availability of sucrose is very limited, lactulosucrose can then act as donor substrate Leuconostoc mesenteroides B?512F ?
-
 ?
sucrose + lactulose
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 Leuconostoc mesenteroides D-fructose + lactulosucrose i.e. beta-D-Gal-(1->4)-beta-D-Fru-(2->1)-alpha-D-Glu, structure determination by NMR, overview ?
sucrose + lactulose
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 Leuconostoc mesenteroides B?512F D-fructose + lactulosucrose i.e. beta-D-Gal-(1->4)-beta-D-Fru-(2->1)-alpha-D-Glu, structure determination by NMR, overview ?
sucrose + [(1->6)-alpha-D-glucosyl]n
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 Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n sucrose is the preferred donor substrate Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
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 ?
sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Leuconostoc mesenteroides B?512F D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
sucrose + [(1->6)-alpha-D-glucosyl]n sucrose is the preferred donor substrate Leuconostoc mesenteroides B?512F D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Leuconostoc mesenteroides

General Information

General Information Comment Organism
additional information lactulosucrose can be an excellent candidate for an emerging bioactive oligosaccharide with potential pharmaceutical and food applications. Having lactulose as a core structure, lactulosucrose might possess the beneficial properties attributed to lactulose, such as the selective stimulation of beneficial bacteria in the large intestine and the regulation of intestinal transit time. Galacto-oligosaccharides derived from lactulose have enhanced functional properties as compared to galactooligosaccharides derived from lactose. Lactulosucrose can exert different or improved biological activities as compared to lactosucrose Leuconostoc mesenteroides