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Literature summary for 2.4.1.5 extracted from

  • Fraga Vidal, R.; Moulis, C.; Escalier, P.; Remaud-Simeon, M.; Monsan, P.
    Isolation of a gene from Leuconostoc citreum B/110-1-2 encoding a novel dextransucrase enzyme (2011), Curr. Microbiol., 62, 1260-1266.
    View publication on PubMed
Search for more literature for 2.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene dsrF, DNA and amino acid sequence determination and analysis, sequence comparison, expression of DSR-F in Escherichia coli strain JM109 Leuconostoc citreum

Protein Variants

Protein Variants Comment Organism
additional information construction of a truncated active variant DSR-F-DELTASPDELTAGBD of 1251 amino acids, with a molecular mass of 145544 Da, the mutant lacks the sequence encoding signal peptide and a portion of the C-terminal domain, i.e. the glucan binding domain Leuconostoc citreum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall DSR-F C-terminal glucan binding domain might be involved in enzyme anchoring at the cell wall surface Leuconostoc citreum 5618
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170448
-
 x * 170448, DSRF, sequence calculation Leuconostoc citreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Leuconostoc citreum dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching ?
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 ?
additional information Leuconostoc citreum B/110-1-2 dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching ?
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 ?

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum D5FS20
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Leuconostoc citreum B/110-1-2 D5FS20
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching Leuconostoc citreum ?
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 ?
additional information the recombinant enzyme catalyzes oligosaccharides synthesis from sucrose as donor and maltose acceptor Leuconostoc citreum ?
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 ?
additional information dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching Leuconostoc citreum B/110-1-2 ?
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 ?
additional information the recombinant enzyme catalyzes oligosaccharides synthesis from sucrose as donor and maltose acceptor Leuconostoc citreum B/110-1-2 ?
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 ?

Subunits

Subunits Comment Organism
? x * 170448, DSRF, sequence calculation Leuconostoc citreum
More the C-terminus, also named glucan binding domain, GBD, begins at position 1171 and is 358 residues in size, the DSR-F C-terminal GBD also contains the unusual repeats named APY 2 times Leuconostoc citreum

Synonyms

Synonyms Comment Organism
DSR-F
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 Leuconostoc citreum

pI Value

Organism Comment pI Value Maximum pI Value
Leuconostoc citreum sequence calculation
-
 5.26

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside-hydrolase family GH70, transglucosidases that produce a-glucans from sucrose Leuconostoc citreum
additional information amino acid residues D557, E595, and D667 are forming the catalytic triad Leuconostoc citreum