expressed in Bacillus megaterium strain MS941 and strain WH320. At high cell density conditions and low growth rates MS941, in contrast to WH320, does not maintain a vegetative growth which is essential for the expression of the foreign dsrS gene by using the xylA promoter. It is conceivable that applications of a promoter which is highly active under nutrient-limited cultivation conditions is necessary, at least for MS941, for the overexpression of recombinant genes in such Bacillus megaterium fed-batch cultivation process |
Leuconostoc mesenteroides |