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Literature summary for 2.4.1.5 extracted from

  • Funane, K.; Ishii, T.; Ono, H.; Kobayashi, M.
    Changes in linkage pattern of glucan products induced by substitution of Lys residues in the dextransucrase (2005), FEBS Lett., 579, 4739-4745.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
S455K produces a glucan with 85% 6-linked glucopyranosyl residues Leuconostoc mesenteroides
T350K produces a glucan with 85% 6-linked glucopyranosyl residues Leuconostoc mesenteroides
T350K/S455K mutant enzyme exhibits a 10fold increase in glucosyltransferase activity over those of the parental DSRS-His6 and its T350K and S455K mutants Leuconostoc mesenteroides
T350K/S455K produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
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NRRL B-512F
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Leuconostoc mesenteroides NRRL B-512F
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NRRL B-512F
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (1,6-alpha-D-glucosyl)n native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues. Mutant enzyme T350K and S455K produce a glucan with 85% 6-linked glucopyranosyl residues. The mutant T350K/S455K produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
?
sucrose + (1,6-alpha-D-glucosyl)n native enzyme produces mainly 6-linked glucopyranosylresidues, while Escherichia coli recombinant enzyme produces a glucan consisting of 70% 6-linked glucopyranosyl residues and 15% 3,6-glucopyranosyl residues. Mutant enzyme T350K and S455K produce a glucan with 85% 6-linked glucopyranosyl residues. The mutant T350K/S455K produces adhesive, water-insoluble glucan with 77% 6-linked glucopyranosyl residues, 8% 3,6-linked glucopyranosyl residues and 4% 2,6-linked glucopyranosyl residues Leuconostoc mesenteroides NRRL B-512F D-fructose + (1,6-alpha-D-glucosyl)n+1
-
?

Synonyms

Synonyms Comment Organism
DSRS
-
Leuconostoc mesenteroides