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Literature summary for 2.4.1.5 extracted from

  • Kralj, S.; van Geel-Schutten, I.G.; Faber, E.J.; van der Maarel, M.J.; Dijkhuizen, L.
    Rational transformation of Lactobacillus reuteri 121 reuteransucrase into a dextransucrase (2005), Biochemistry, 44, 9206-9216.
    View publication on PubMed
Search for more literature for 2.4.1.5

Protein Variants

Protein Variants Comment Organism
N1134S/N1135E/S1136V mutation converts glucosyltransferase from a mainly alpha-(1,4) (about 45%, reuteran) to a mainly alpha-(1,6) (about 80%, dextran) synthesizing enzyme Limosilactobacillus reuteri
P1026V/I1029V/N1134S/N1135E/S1136V mutant enzyme synthesizes an alpha-glucan containing only a very small percentage of alpha-(1,4) glucosidic linkages (about 5%) and a further increased percentage of alpha-(1,6) glucosidic linkages (about 85%) Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri
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Limosilactobacillus reuteri 121
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information triple mutation N1134S/N1135E/S1136V converts glucosyltransferase from a mainly alpha-(1,4) (about 45%, reuteran) to a mainly alpha-(1,6) (about 80%, dextran) synthesizing enzyme. Mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V synthesizes an alpha-glucan containing only a very small percentage of alpha-(1,4) glucosidic linkages (about 5%) and a further increased percentage of alpha-(1,6) glucosidic linkages (about 85%) Limosilactobacillus reuteri ?
-
 ?
additional information triple mutation N1134S/N1135E/S1136V converts glucosyltransferase from a mainly alpha-(1,4) (about 45%, reuteran) to a mainly alpha-(1,6) (about 80%, dextran) synthesizing enzyme. Mutant enzyme P1026V/I1029V/N1134S/N1135E/S1136V synthesizes an alpha-glucan containing only a very small percentage of alpha-(1,4) glucosidic linkages (about 5%) and a further increased percentage of alpha-(1,6) glucosidic linkages (about 85%) Limosilactobacillus reuteri 121 ?
-
 ?