Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermosipho africanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
cellopentaose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
0.062 | - |
cellotetraose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
0.094 | - |
cellotriose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
0.12 | - |
cellobiose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
1.57 | - |
alpha-D-glucose 1-phosphate | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
2.59 | - |
cellopentaose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
3.7 | - |
cellotetraose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
4.05 | - |
cellotriose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
7.39 | - |
cellobiose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
14.3 | - |
alpha-D-glucose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
25.7 | - |
alpha-D-xylose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosipho africanus | B7IED6 | - |
- |
Thermosipho africanus TCF52B | B7IED6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + alpha-D-glucose | - |
Thermosipho africanus | phosphate + cellobiose | - |
? | |
alpha-D-glucose 1-phosphate + alpha-D-xylose | - |
Thermosipho africanus | phosphate + alpha-D-Glc-(1->4)-alpha-D-Xyl | - |
? | |
alpha-D-glucose 1-phosphate + cellobiose | - |
Thermosipho africanus | phosphate + cellotriose | - |
r | |
alpha-D-glucose 1-phosphate + cellobiose | - |
Thermosipho africanus TCF52B | phosphate + cellotriose | - |
r | |
alpha-D-glucose 1-phosphate + cellopentaose | - |
Thermosipho africanus | phosphate + cellohexaose | - |
r | |
alpha-D-glucose 1-phosphate + cellotetraose | - |
Thermosipho africanus | phosphate + cellopentaose | - |
r | |
alpha-D-glucose 1-phosphate + cellotetraose | - |
Thermosipho africanus TCF52B | phosphate + cellopentaose | - |
r | |
alpha-D-glucose 1-phosphate + cellotriose | - |
Thermosipho africanus | phosphate + cellotetraose | - |
r | |
alpha-D-glucose 1-phosphate + cellotriose | - |
Thermosipho africanus TCF52B | phosphate + cellotetraose | - |
r | |
beta-D-Xyl-(1->4)-beta-D-Glc-(1->4)-D-Glc + alpha-D-xylose 1-phosphate | - |
Thermosipho africanus | beta-D-Xyl-(1->4)-beta-D-Xyl-(1->4)-beta-D-Glc-(1->4)-D-Glc + phosphate | - |
? | |
cellobiose + phosphate | - |
Thermosipho africanus | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellobiose + phosphate | - |
Thermosipho africanus TCF52B | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
cellopentaose + phosphate | - |
Thermosipho africanus | alpha-D-glucose 1-phosphate + cellotetraose | - |
? | |
cellotetraose + phosphate | - |
Thermosipho africanus | alpha-D-glucose 1-phosphate + cellotriose | - |
? | |
cellotriose + phosphate | - |
Thermosipho africanus | alpha-D-glucose 1-phosphate + cellobiose | - |
? | |
additional information | enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than two as a glucose acceptor. Cellotetraose and cellopentaose are the best substrates for the phosphorolytic and reverse synthetic reactions, respectively. It prefes cellobiose and cellodextrins to glucose in the synthetic direction | Thermosipho africanus | ? | - |
- |
|
additional information | enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than two as a glucose acceptor. Cellotetraose and cellopentaose are the best substrates for the phosphorolytic and reverse synthetic reactions, respectively. It prefes cellobiose and cellodextrins to glucose in the synthetic direction | Thermosipho africanus TCF52B | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE | Thermosipho africanus |
Synonyms | Comment | Organism |
---|---|---|
THA_1941 | - |
Thermosipho africanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermosipho africanus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
30 min, 80% residual activity | Thermosipho africanus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
cellobiose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
0.28 | - |
cellotriose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
0.34 | - |
cellopentaose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
0.49 | - |
cellotetraose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
1.37 | - |
alpha-D-xylose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
7.61 | - |
alpha-D-glucose 1-phosphate | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
30.3 | - |
alpha-D-glucose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
361 | - |
cellobiose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
527 | - |
cellopentaose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
603 | - |
cellotriose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
612 | - |
cellotetraose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Thermosipho africanus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
alpha-D-xylose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
1.92 | - |
cellobiose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
2.12 | - |
alpha-D-glucose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
2.98 | - |
cellotriose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
4.85 | - |
alpha-D-glucose 1-phosphate | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
5.67 | - |
cellopentaose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
7.91 | - |
cellotetraose | phosphoroyltic reaction, pH 7.5, 60°C | Thermosipho africanus | |
48.87 | - |
cellobiose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
148.9 | - |
cellotriose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
165.5 | - |
cellotetraose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus | |
203.5 | - |
cellopentaose | synthetic reaction, pH 7.5, 60°C | Thermosipho africanus |