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Literature summary for 2.4.1.49 extracted from

  • Wu, Y.; Mao, G.; Fan, H.; Song, A.; Zhang, Y.P.; Chen, H.
    Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity (2017), Sci. Rep., 7, 4849 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermosipho africanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
cellopentaose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
0.062
-
cellotetraose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
0.094
-
cellotriose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
0.12
-
cellobiose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
1.57
-
alpha-D-glucose 1-phosphate synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.59
-
cellopentaose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
3.7
-
cellotetraose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
4.05
-
cellotriose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
7.39
-
cellobiose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
14.3
-
alpha-D-glucose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
25.7
-
alpha-D-xylose synthetic reaction, pH 7.5, 60°C Thermosipho africanus

Organism

Organism UniProt Comment Textmining
Thermosipho africanus B7IED6
-
-
Thermosipho africanus TCF52B B7IED6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + alpha-D-glucose
-
Thermosipho africanus phosphate + cellobiose
-
?
alpha-D-glucose 1-phosphate + alpha-D-xylose
-
Thermosipho africanus phosphate + alpha-D-Glc-(1->4)-alpha-D-Xyl
-
?
alpha-D-glucose 1-phosphate + cellobiose
-
Thermosipho africanus phosphate + cellotriose
-
r
alpha-D-glucose 1-phosphate + cellobiose
-
Thermosipho africanus TCF52B phosphate + cellotriose
-
r
alpha-D-glucose 1-phosphate + cellopentaose
-
Thermosipho africanus phosphate + cellohexaose
-
r
alpha-D-glucose 1-phosphate + cellotetraose
-
Thermosipho africanus phosphate + cellopentaose
-
r
alpha-D-glucose 1-phosphate + cellotetraose
-
Thermosipho africanus TCF52B phosphate + cellopentaose
-
r
alpha-D-glucose 1-phosphate + cellotriose
-
Thermosipho africanus phosphate + cellotetraose
-
r
alpha-D-glucose 1-phosphate + cellotriose
-
Thermosipho africanus TCF52B phosphate + cellotetraose
-
r
beta-D-Xyl-(1->4)-beta-D-Glc-(1->4)-D-Glc + alpha-D-xylose 1-phosphate
-
Thermosipho africanus beta-D-Xyl-(1->4)-beta-D-Xyl-(1->4)-beta-D-Glc-(1->4)-D-Glc + phosphate
-
?
cellobiose + phosphate
-
Thermosipho africanus alpha-D-glucose 1-phosphate + D-glucose
-
?
cellobiose + phosphate
-
Thermosipho africanus TCF52B alpha-D-glucose 1-phosphate + D-glucose
-
?
cellopentaose + phosphate
-
Thermosipho africanus alpha-D-glucose 1-phosphate + cellotetraose
-
?
cellotetraose + phosphate
-
Thermosipho africanus alpha-D-glucose 1-phosphate + cellotriose
-
?
cellotriose + phosphate
-
Thermosipho africanus alpha-D-glucose 1-phosphate + cellobiose
-
?
additional information enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than two as a glucose acceptor. Cellotetraose and cellopentaose are the best substrates for the phosphorolytic and reverse synthetic reactions, respectively. It prefes cellobiose and cellodextrins to glucose in the synthetic direction Thermosipho africanus ?
-
-
additional information enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than two as a glucose acceptor. Cellotetraose and cellopentaose are the best substrates for the phosphorolytic and reverse synthetic reactions, respectively. It prefes cellobiose and cellodextrins to glucose in the synthetic direction Thermosipho africanus TCF52B ?
-
-

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Thermosipho africanus

Synonyms

Synonyms Comment Organism
THA_1941
-
Thermosipho africanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermosipho africanus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
30 min, 80% residual activity Thermosipho africanus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
cellobiose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
0.28
-
cellotriose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
0.34
-
cellopentaose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
0.49
-
cellotetraose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
1.37
-
alpha-D-xylose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
7.61
-
alpha-D-glucose 1-phosphate synthetic reaction, pH 7.5, 60°C Thermosipho africanus
30.3
-
alpha-D-glucose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
361
-
cellobiose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
527
-
cellopentaose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
603
-
cellotriose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
612
-
cellotetraose synthetic reaction, pH 7.5, 60°C Thermosipho africanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Thermosipho africanus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
alpha-D-xylose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
1.92
-
cellobiose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.12
-
alpha-D-glucose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.98
-
cellotriose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
4.85
-
alpha-D-glucose 1-phosphate synthetic reaction, pH 7.5, 60°C Thermosipho africanus
5.67
-
cellopentaose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
7.91
-
cellotetraose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
48.87
-
cellobiose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
148.9
-
cellotriose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
165.5
-
cellotetraose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
203.5
-
cellopentaose synthetic reaction, pH 7.5, 60°C Thermosipho africanus