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Literature summary for 2.4.1.49 extracted from

  • Zhong, C.; Luley-Goedl, C.; Nidetzky, B.
    Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase (2019), Biotechnol. Bioeng., 116, 2146-2155 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis biocatalytic production of cellodextrins with a degree of polymerization. Kinetic and thermodynamic restrictions upon alpha-Glc1-P utilization (200 mM; 45°C, pH 7.0) are effectively overcome by in situ removal of the phosphate released via precipitation with Mg2+. The product's degree of polymerization is controlled by the molar ratio of glucose/alpha-Glc1-P used in the reaction. In optimized conversion, 36 g/l soluble cellodextrins are obtained. By keeping the glucose concentration low, the reaction is shifted completely towards insoluble product formation Thermoclostridium stercorarium

Organism

Organism UniProt Comment Textmining
Thermoclostridium stercorarium P77846
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Synonyms

Synonyms Comment Organism
CepB
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Thermoclostridium stercorarium