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Literature summary for 2.4.1.49 extracted from

  • Ye, X.; Zhu, Z.; Zhang, C.; Zhang, Y.H.
    Fusion of a family 9 cellulose-binding module improves catalytic potential of Clostridium thermocellum cellodextrin phosphorylase on insoluble cellulose (2011), Appl. Microbiol. Biotechnol., 92, 551-560.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein and His-tagged fusion proteins harboring a cellulose binding module expressed in Escherichia coli BL21 Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus
0.53
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus
0.59
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
0.62
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
0.76
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
0.78
-
cellobiose synthesis direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
1.08
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
1.29
-
celloheptaose synthesis direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
1.46
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
1.53
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
1.56
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus
1.82
-
celloheptaose degradation direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
1.93
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
2.71
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
5.24
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
x * 115000, SDS-PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + alpha-D-glucose 1-phosphate weak activities on celloheptaose and insoluble regenerated amorphous cellulose Acetivibrio thermocellus cellotriose + phosphate
-
r
celloheptaose + alpha-D-glucose 1-phosphate weak activities on celloheptaose and insoluble regenerated amorphous cellulose Acetivibrio thermocellus cellooctaose + phosphate
-
r

Subunits

Subunits Comment Organism
? x * 115000, SDS-PAGE Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
cellodextrin phosphorylase
-
Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
wild type and fusion proteins Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.08
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
1.37
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
1.83
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
2.07
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus
2.08
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
2.16
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
2.25
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus
2.55
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
2.67
-
cellobiose synthesis direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
2.7
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
2.98
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
3.11
-
celloheptaose synthesis direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
3.77
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
4.14
-
celloheptaose degradation direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
4.53
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
wild type and fusion proteins Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.41
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
0.56
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
0.9
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
1
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
2.04
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
2.27
-
celloheptaose degradation direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
2.36
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
2.41
-
celloheptaose synthesis direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
2.73
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30°C Acetivibrio thermocellus
2.9
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus
3.1
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30°C Acetivibrio thermocellus
3.42
-
cellobiose synthesis direction, wild type protein, pH 7.2, 30°C Acetivibrio thermocellus
3.9
-
cellobiose synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus
6.08
-
celloheptaose degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30°C Acetivibrio thermocellus
7.76
-
celloheptaose synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30°C Acetivibrio thermocellus